CASP1 ELISA Kit (Pig) (OKCA02485)

Catalog No: OKCA02485

Size:96 Wells

Pig CASP1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Pig
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  Neat	89	83-100
  1:2	90	80-99
  1:4	95	84-100
  1:8	95	83-100

• ELISA Kit Principle: Aviva Systems Biology CASP1 ELISA Kit (Pig) (OKCA02485) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CASP1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CASP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard CASP1 captured in the well.
• ELISA Kit Range: 25 - 1,600 pg/mL
• ELISA Kit Recovery:

  Sample Type	Mean Recovery (%)	Range (%)
  Serum (n=5)	93	82-97
  EDTA Plasma (n=4)	93	86-100

• ELISA Kit Reproducibility: Mean Intra-Assay: CV <12% (n=20)
  Mean Inter-Assay: CV <15% (n=24)
• ELISA Kit Component:
  • CASP1 Microplate: 96 Wells (12 x 8 Well strips)
  • CASP1 Lyophilized Standard: 2
  • 100X Biotinylated CASP1 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates
• Sensitivity: 6.25 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: CASP1
• Alias Symbols: ICE, IL1BC, P45, CASP1 nirs variant 1, IL1B-convertase, caspase 1, caspase 1, apoptosis-related cysteine protease (interleukin 1, beta, convertase), caspase-1, interleukin 1-B converting enzyme, interleuk
• NCBI Gene Id: 397319
• Protein Name: Caspase-1
• Description of Target: Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.
• Uniprot ID: Q9N2I1
• Protein Accession #: NP_999327
• Nucleotide Accession #: NM_214162