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Catalog No: OKCD02418
Size:96WELLS
Price: $650.00
SKU
OKCD02418
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Datasheets/ManualsPrintable datasheet for CASP1 ELISA Kit (Mouse) (OKCD02418)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)82-101%81-93%87-101%89-101%
EDTA Plasma (n=5)87-96%98-105%91-102%91-105%
Heparin Plasma (n=5)80-104%96-105%98-105%96-103%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Caspase 1 (CASP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Caspase 1 (CASP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Caspase 1 (CASP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Caspase 1 (CASP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range31.2-2,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)88-9591
EDTA Plasma (n=5)93-10298
Heparin Plasma(n=5)79-9082
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Caspase 1 (CASP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Caspase 1 (CASP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CASP1 Microplate96 Wells (12 x 8 Well strips)
CASP1 Lyophilized Standard2
100X Biotinylated CASP1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernatant and other biological fluids
Sensitivity13.5 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Caspase 1 (CASP1). No significant cross-reactivity or interference between Caspase 1 (CASP1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolCasp1
Gene Full Namecaspase 1
Alias SymbolsCasp;CASP-1;caspase-1;IC;ICE;Il1;IL-1 beta-converting enzyme;IL-1B converting enzyme;Il1bc;IL-1BC;interleukin-1 beta convertase;interleukin-1 beta-converting enzyme;p45.
NCBI Gene Id12362
Protein NameCaspase-1
Description of TargetThiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis (By similarity). Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive (PubMed:28314590).
Uniprot IDP29452
Protein Accession #NP_033937.2
Nucleotide Accession #NM_009807.2
Protein Size (# AA)402
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