CASP1 ELISA Kit (Human) : 96 Wells (OKEH01146)

Catalog No: OKEH01146

Size:96 Wells

Human CASP1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology CASP1 ELISA Kit (Human) (OKEH01146) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CASP1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CASP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CASP1 captured in well.
• ELISA Kit Range: 15.6 - 1000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 6.1% (n = 20)
  Mean Inter-assay CV%: < 8.3% (n = 20)
• ELISA Kit Component:
  • CASP1 Microplate: 96 Wells (12 x 8 Well strips)
  • CASP1 Lyophilized Standard: 2
  • 100X Biotinylated CASP1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 10 pg/mL
• Specificity: Natural and recombinant Human Caspase-1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CASP1
• Gene Full Name: caspase 1
• Alias Symbols: ICE, P45, IL1BC
• NCBI Gene Id: 834
• Protein Name: Caspase-1
• Description of Target: This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms.
• Uniprot ID: P29466
• Protein Accession #: NP_001214.1
• Nucleotide Accession #: NM_001223.4
• Protein Size (# AA): 404
• Molecular Weight: 45 kDa