CARTPT ELISA Kit (Rat) (OKCD06259)

Catalog No: OKCD06259

Size:96WELLS

CARTPT ELISA Kit (Rat) (OKCD06259) Standard Curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cocaine And Amphetamine Regulated Transcript (CART). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cocaine And Amphetamine Regulated Transcript (CART). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cocaine And Amphetamine Regulated Transcript (CART), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cocaine And Amphetamine Regulated Transcript (CART) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.62-1000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cocaine And Amphetamine Regulated Transcript (CART) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cocaine And Amphetamine Regulated Transcript (CART) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CARTPT Microplate: 96 Wells (12 x 8 Well strips)
  • CARTPT Lyophilized Standard: 2
  • 100X CARTPT HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 5.9pg/mL

Target Info

• Gene Symbol: Cartpt
• Gene Full Name: CART prepropeptide
• Alias Symbols: Cart;cocaine and amphetamine regulated transcript;cocaine- and amphetamine-regulated transcript protein.
• NCBI Gene Id: 29131
• Protein Name: Cocaine- and amphetamine-regulated transcript protein
• Description of Target: Satiety factor closely associated with the actions of leptin and neuropeptide y; this anorectic peptide inhibits both normal and starvation-induced feeding and completely blocks the feeding response induced by neuropeptide Y and regulated by leptin in the hypothalamus.
• Uniprot ID: P49192
• Protein Accession #: NP_058806.1
• Nucleotide Accession #: NM_017110.1