CARTPT ELISA Kit (Bovine) : 96 Wells (OKEH00540)

Catalog No: OKEH00540

Size:96T

Bovine CARTPT ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology CARTPT ELISA Kit (Bovine) (OKEH00540) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Bovine CARTPT antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated CARTPT and incubated. The CARTPT found in the sample or standards competes with the biotinylated CARTPT for limited binding sites on the immobilized anti-Bovine CARTPT antibody. Excess unbound biotinylated CARTPT and sample or standard CARTPT is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated CARTPT captured in the well and inversely proportional to the amount of CARTPT which was contained in the sample or standard.
• ELISA Kit Range: 0.469-30ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.2% (n = 20)
  Mean Inter-assay CV%: < 8.3% (n = 20)
• ELISA Kit Component:
  • Anti-CARTPT Microplate: 96 Wells (12 x 8 Well strips)
  • CARTPT Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X CARTPT-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.226 ng/mL
• Specificity: Natural and recombinant Bovine Cocaine- and amphetamine-regulated transcript protein
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CARTPT
• Gene Full Name: CART prepropeptide
• Alias Symbols: CART;cocaine and amphetamine responsive transcript;cocaine- and amphetamine-regulated transcript protein.
• NCBI Gene Id: 493726
• Protein Name: Q68RJ9
• Description of Target: Cocaine- and amphetamine-regulated transcript protein
• Uniprot ID: https://www.uniprot.org/uniprot/Q68RJ9
• Protein Accession #: NP_001007821.1
• Nucleotide Accession #: NM_001007820.3
• Protein Size (# AA): 116
• Molecular Weight: 13 kDa