Cardiac Troponin I Recombinant Antibody [scFv 180] (OAAV00763)

Catalog No: OAAV00763

Size:200UG

Western Blot using anti-Cardiac Troponin I antibody scFv 180 Human heart lysate (35µg protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric mouse IgG1 version of scFv 180 at 0.001 µg/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted running size for Cardiac Troponin I is 24.0 kDa. Successfully detected Cardiac Troponin I in human heart lysate.

Immunofluorescence staining of fixed HeLa cells with anti-Cardiac Troponin I antibody scFv 180 (OAAV00763) Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of scFv 180 (OAAV00763) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom OAAV00763, DAPI, merged channels and an isotype control.

Immunofluorescence staining of fixed HeLa cells with anti-Cardiac Troponin I antibody scFv 180 (OAAV00763) Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of scFv 180 (OAAV00763) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom OAAV00763, DAPI, merged channels and an isotype control.

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human
• Product Format: PBS with 0.02% Proclin 300.
• Clonality: Recombinant Monoclonal
• Clone: scFv 180
• Isotype: IgG1, lambda
• Host: Mouse
• Application: Surface plasmon resonance,
  Western blot
• Reconstitution and Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Immunogen: Cardiac Troponin I peptide (KISASRKLQLKT).
• Purification: Purified
• Specificity: This antibody binds specificially to the cardiac Troponin I Peptide KISASRKLQLKT. This epitope is 100% conserved among a great variety of mammalian species, including most monkeys, Squirrel, Hamster, Mouse, Rat, Rabbit, Whales, Mole-Rat, Antelope and several other mammals.
• Description: This full-length, chimeric mouse antibody was made using the variable domain sequences of the original Chicken scFv format, for improved compatibility with existing reagents, assays and techniques.

Target Info

• Gene Symbol: TNNI3
• Gene Full Name: troponin I3, cardiac type
• Alias Symbols: Cardiac troponin I;cardiomyopathy, dilated 2A (autosomal recessive);CMD1FF;CMD2A;CMH7;cTnI;RCM1;TNNC1;troponin I type 3 (cardiac);troponin I, cardiac muscle.
• NCBI Gene Id: 7137
• Protein Name: Troponin I, cardiac muscle
• Description of Target: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
• Uniprot ID: P19429
• Protein Accession #: NP_000354.4
• Nucleotide Accession #: NM_000363.4