CAPZB ELISA Kit (Human) (OKCD09201)

Catalog No: OKCD09201

Size:96WELLS

CAPZB ELISA Kit (Human) (OKCD09201) Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Capping Protein Muscle Z Line Beta (CAPZb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Capping Protein Muscle Z Line Beta (CAPZb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Capping Protein Muscle Z Line Beta (CAPZb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Capping Protein Muscle Z Line Beta (CAPZb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Capping Protein Muscle Z Line Beta (CAPZb) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Capping Protein Muscle Z Line Beta (CAPZb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CAPZB Microplate: 96 Wells (12 x 8 Well strips)
  • CAPZB Lyophilized Standard: 2
  • 100X CAPZB HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.059ng/mL

Target Info

• Gene Symbol: CAPZB
• Gene Full Name: capping actin protein of muscle Z-line beta subunit
• Alias Symbols: CAPB, CAPZ, CAPPB
• NCBI Gene Id: 832
• Protein Name: F-actin-capping protein subunit beta
• Description of Target: This gene encodes the beta subunit of the barbed-end actin binding protein, which belongs to the F-actin capping protein family. The capping protein is a heterodimeric actin capping protein that blocks actin filament assembly and disassembly at the fast growing (barbed) filament ends and functions in regulating actin filament dynamics as well as in stabilizing actin filament lengths in muscle and nonmuscle cells. A pseudogene of this gene is located on the long arm of chromosome 2. Multiple alternatively spliced transcript variants encoding different isoforms have been found.
• Uniprot ID: P47756
• Protein Accession #: NP_001193469.1
• Nucleotide Accession #: NM_001206540.2