CAPN5 ELISA Kit (Rat) (OKEH03490)

Catalog No: OKEH03490

Size:96T

Rat CAPN5 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology CAPN5 ELISA Kit (Rat) (OKEH03490) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human Capn5 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Capn5 and incubated. The Capn5 found in the sample or standards competes with the biotinylated Capn5 for limited binding sites on the immobilized anti-human Capn5 antibody. Excess unbound biotinylated Capn5 and sample or standard Capn5 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Capn5 captured in the well and inversely proportional to the amount of Capn5 which was contained in the sample or standard.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Component:
  • Anti-Capn5 Microplate: 96 Wells (12 x 8 Well strips)
  • Capn5 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Capn5-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.398 ng/mL
• Specificity: Natural and recombinant Rat Calpain-5
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: CAPN5
• Gene Full Name: calpain 5
• Alias Symbols: Htra3
• NCBI Gene Id: 171495
• Protein Name: Q8R4C0
• Description of Target: Calpain-5
• Uniprot ID: https://www.uniprot.org/uniprot/Q8R4C0
• Protein Accession #: NP_604456.2
• Nucleotide Accession #: NM_134461.2
• Protein Size (# AA): 640
• Molecular Weight: 73 kDa