Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any help please email info@avivasysbio.com

Catalog No: OKEH03489
Size:96T
Price: $525.00
SKU
OKEH03489
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CAPN3 ELISA Kit (Rat) (OKEH03489) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Capn3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Capn3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Capn3 captured in well.
ELISA Kit Range0.625-40ng/mL
ELISA Kit Component
ComponentAmount
Capn3 Microplate96 Wells (12 x 8 Well strips)
Capn3 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Capn3 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.322 ng/mL
SpecificityNatural and recombinant Rat Calpain-3
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCapn3
Gene Full Namecalpain 3
Alias Symbolscalcium-activated neutral proteinase 3;calpain L3;calpain p94;calpain-3;CANP 3;Lp82;Lp84;Lp85;muscle-specific calcium-activated neutral protease 3;p94.
NCBI Gene Id29155
Protein NameP16259
Description of TargetCalpain-3
Uniprot IDhttps://www.uniprot.org/uniprot/P16259
Protein Accession #NP_058813.1
Nucleotide Accession #NM_017117.1
Protein Size (# AA)821
Molecular Weight94 kDa
Write Your Own Review
You're reviewing:CAPN3 ELISA Kit (Rat) (OKEH03489)
Your Rating
We found other products you might like!