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Catalog No: OPPA01245 (Formerly GWB-33B130)
Size:2ug
Price: $324.00
SKU
OPPA01245
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CAPN2 Protein (OPPA01245)

Datasheets/ManualsPrintable datasheet for OPPA01245
Product Info
Product Format50mM Imidazole-HCl, 0.2mM EDTA, 1mM DTT and 50% glycerol, pH 7.4. Physical appearance: Sterile Filtered colorless solution.
HostBovine Myocardium
ApplicationWB, IHC, ELISA
::Product Introduction: Calpain's activity is attributed to two main isoforms: *-calpain and m-calpain, which are ubiquitously expressed proteases implicated in cellular migration, cell cycle progression, degenerative processes and cell death. These heterodimeric enzymes are composed of distinct catalytic subunits, encoded by Capn1 (*-calpain) or Capn2 (m-calpain), and a common regulatory subunit encoded by Capn4. Calpain2 (M-calpain) is believed to be membrane bound and functions at the trailing edge of the migrating cell to cleave the integrins in response to growth factor receptor signals. PKA functions to down regulate or inhibit calpain2.
Product Description: CAPN2 consists of an 80 kDa large subunit and a 30 kDa small subunit. CAPN2 was purified by sequential chromatography through DEAE-Sepharose, A 1.5m Bio-Gel, and Phenyl-Sepharose CL-4B columns.
Reconstitution and StorageCAPN2 although stable at 10C for 1 week, should be stored desiccated below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles.
PurityGreater than 90% as determined by SDS-PAGE.
Application InfoThis protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection.For adsorption we suggest the following procedureA- Dilute 1 ul of the antiserum against m-calpain in 1ml of the usual buffer for immunohistochemistry (final dilution 1:1'000).B- Add 1 ug of protein to 1ml of the diluted antibody solution and mix well.C- Incubate for at least 6 hours in the cold.D- Apply to tissue-sections and incubate for 3 days.E - Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB).As a result, the immunostaining should be strongly reduced or even completely prevented.
Gene SymbolbCAPN2
Alias SymbolsCalpain-2 catalytic subunit, EC 3.4.22.53, Calpain-2 large subunit, Calcium-activated neutral proteinase 2, CANP 2, Calpain M-type, M-calpain, Millimolar-calpain, CAPN2, CAPN2, Bovine Calpain-2 Catalytic Subunit
NCBI Gene Id281662
Protein NameCalpain-2 catalytic subunit
Description of TargetBovine Calpain-2 Catalytic Subunit
Uniprot IDQ27971
Protein Accession #NP_001096556.1
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