CAPN2 Antibody - middle region (OAGA03898)

Catalog No: OAGA03898

Size:100 ul

Sample (30 ug of whole cell lysate)
A: 293T
7.5% SDS PAGE
diluted at 1:1000

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Calpain 2 antibody at 1:500 dilution.

Calpain 2 antibody [N2C1-2], Internal detects Calpain 2 protein at cytoplasm in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Calpain 2 antibody [N2C1-2], Internal diluted at 1:500.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IHC-P, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Calpain 2. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.47 mg/ml
• Preservative: 0.01% Thimerosal
• Application Info: Suggested starting dilutions are as follows: IHC-P: 1:100-1:1000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: CAPN2
• Gene Full Name: calpain 2
• Alias Symbols: CANP2, mCANP, CANPL2, CANPml
• NCBI Gene Id: 824
• Protein Name: calpain-2 catalytic subunit
• Description of Target: The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene.
• Uniprot ID: P17655
• Protein Accession #: NP_001139540.1
• Nucleotide Accession #: NM_001146068.1