CAMP ELISA Kit (Human) (OKCA00619)

Catalog No: OKCA00619

Size:96 Wells

Human Antibacterial Peptide LL-37 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean n=4 (%)	Range (%)
  1:5	85	80-92
  1:10	98	91-105
  1:20	98	92-110
  1:40	93	86-98

• ELISA Kit Principle: Aviva Systems Biology Antibacterial Peptide LL-37 ELISA Kit (Human) (OKCA00619) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Antibacterial Peptide LL-37 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Antibacterial Peptide LL-37 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Antibacterial Peptide LL-37 captured in the well.
• ELISA Kit Range: 0.156 ng/mL-10 ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	94	89-98
  EDTA Plasma (n=4)	104	92-112

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level Antibacterial Peptide LL-37 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level Antibacterial Peptide LL-37 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • Antibacterial Peptide LL-37 Microplate: 96 Wells (12 x 8 Well strips)
  • Antibacterial Peptide LL-37 Lyophilized Standard: 2
  • 100X Biotinylated Antibacterial Peptide LL-37 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 0.039 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CAMP
• Gene Full Name: Cathelicidin antimicrobial peptide
• Alias Symbols: LL37, CAP18, CRAMP, HSD26, CAP-18, FALL39, FALL-39
• NCBI Gene Id: 820
• Protein Name: Cathelicidin antimicrobial peptide
• Description of Target: Binds to bacterial lipopolysaccharides (LPS), has antibacterial activity.
• Uniprot ID: P49913
• Protein Accession #: NP_004336.3
• Nucleotide Accession #: NM_004345.4