CAMP ELISA Kit (Human) : 96 Wells (OKEH00728)

Catalog No: OKEH00728 (Formerly GWB-AO5IR0)

Size:96T

Human CAMP ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology CAMP ELISA Kit (Human) (OKEH00728) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for CAMP has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CAMP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CAMP captured in the well.
• ELISA Kit Range: 125-8000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 81%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.2% (n = 20)
  Mean Inter-assay CV%: < 6.9% (n = 24)
• ELISA Kit Component:
  • Anti-CAMP Microplate: 96 Wells (12 x 8 Well strips)
  • CAMP Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X CAMP Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 44 pg/mL
• Specificity: Natural and recombinant Human Cathelicidin antimicrobial peptide
• Publications:

  Association of vitamin D3, VDR gene polymorphisms, and LL-37 with a clinical form of Chagas Disease. Rev Soc Bras Med Trop. 52, e20190133 (2019). 31508781

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA
• Description:
  View product citations for CAMP ELISA Kit (Human) : 96 Wells (OKEH00728) on CiteAb

Target Info

• Gene Symbol: CAMP
• Gene Full Name: cathelicidin antimicrobial peptide
• Alias Symbols: 18 kDa cationic antimicrobial protein;CAP18;CAP-18;cathelicidin antimicrobial peptide;CRAMP;epididymis secretory sperm binding protein;FALL39;FALL-39;HSD26;LL37.
• NCBI Gene Id: 820
• Protein Name: P49913
• Description of Target: Cathelicidin antimicrobial peptide
• Uniprot ID: https://www.uniprot.org/uniprot/P49913
• Protein Accession #: NP_004336.3
• Nucleotide Accession #: NM_004345.4
• Protein Size (# AA): 170
• Molecular Weight: 19 kDa