CAMKK2 Antibody - N-terminal region (ARP53184_P050)

Catalog No: ARP53184_P050

Host: Rabbit
Target Name: CAMKK2
Sample Tissue: Human A549 Whole Cell
Antibody Dilution: 3ug/ml

Host: Rabbit
Target Name: CAMKK2
Sample Type: Human Adult Placenta
Antibody Dilution: 1.0ug/ml

Host: Rabbit
Target Name: CAMKK2
Sample Type: Human Fetal Liver
Antibody Dilution: 1.0ug/ml

WB Suggested Anti-CAMKK2 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: 721_B cell lysateThere is BioGPS gene expression data showing that CAMKK2 is expressed in 721_B

Host: Rabbit
Target Name: CAMKK2
Sample Type: Human Hela
Antibody Dilution: 1.0ug/mlThere is BioGPS gene expression data showing that CAMKK2 is expressed in HeLa

Host: Rabbit
Target Name: CAMKK2
Sample Type: Human HepG2
Antibody Dilution: 1.0ug/mlThere is BioGPS gene expression data showing that CAMKK2 is expressed in HepG2

Host: Rabbit
Target Name: CAMKK2
Sample Type: Human Jurkat
Antibody Dilution: 1.0ug/mlCAMKK2 is supported by BioGPS gene expression data to be expressed in Jurkat

Host: Rabbit
Target Name: CAMKK2
Sample Type: Human MCF7
Antibody Dilution: 1.0ug/mlCAMKK2 is supported by BioGPS gene expression data to be expressed in MCF7

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Pig, Rabbit, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CAMKK2
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 93%; Dog: 93%; Guinea Pig: 86%; Human: 100%; Mouse: 86%; Pig: 93%; Rabbit: 93%; Rat: 86%; Zebrafish: 79%
• Peptide Sequence: Synthetic peptide located within the following region: GGLAAGGSLDMNGRCICPSLPYSPVSSPQSSPRLPRRPTVESHHVSITGM
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-CAMKK2 (ARP53184_P050) antibody is Catalog # AAP53184 (Previous Catalog # AAPP36322)
• Sample Type Confirmation:

  There is BioGPS gene expression data showing that CAMKK2 is expressed in 721_B, Hela, HepG2

  CAMKK2 is supported by BioGPS gene expression data to be expressed in Jurkat, MCF7

Target Info

• Reference: Erhardt,A., J Affect Disord 101 (1-3), 159-168 (2007)
• Gene Symbol: CAMKK2
• Gene Full Name: Calcium/calmodulin-dependent protein kinase kinase 2, beta
• Alias Symbols: CAMKK, CAMKKB
• NCBI Gene Id: 10645
• Protein Name: Calcium/calmodulin-dependent protein kinase kinase 2
• Description of Target: CAMKK2 is a calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Isoform 1, isoform 2 and isoform 3 phosphorylate CAMK1 and CAMK4. Isoform 3 phosphorylates CAMK1D. Isoform 4, isoform 5 and isoform 6 lacking part of the calmodulin-binding domain are inactive. CAMKK2 seems to be involved in hippocampal activation of CREB1.The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade by phosphorylating the downstream kinases CaMK1 and CaMK4. Seven transcript variants encoding six distinct isoforms have been identified for this gene. Additional splice variants have been described but their full-length nature has not been determined. The identified isoforms exhibit a distinct ability to undergo autophosphorylation and to phosphorylate the downstream kinases.
• Uniprot ID: Q96RR4
• Protein Accession #: NP_705720
• Nucleotide Accession #: NM_153500
• Protein Size (# AA): 498
• Molecular Weight: 55kDa
• Protein Interactions: HSP90AA1; APP; UBC; NEDD4; CEP63; ESYT2; FANCI; OBSL1; ATG4B; XPOT; GCN1L1; IQGAP2; SMC3; SLC25A11; SMC1A; PRKDC; PRKAG1; PRKAA2; IRAK1; FLNC; FLNA; MAPK14; CALM1; CAMK4; CAMK1; PRKACA; SGK1;