CaMKII Antibody : FITC (OASE00039-FITC)

Catalog No: OASE00039-FITC

Size:100ug

Product Info

• Predicted Species Reactivity: Rat
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer.
• Clonality: Monoclonal
• Clone: 22B1
• Isotype: IgG1
• Host: Mouse
• Conjugation: FITC: Fluorescein Isothiocyanate
• Application: ELISA, IF, IP, WB
• Additional Information: Background Info: Detects phosphorylated CaMKII from rat tissues. This antibody is specific for alpha and beta subunits of CaMKII only when they are phosphorylated at Thr-286/287 (in beta).
  Scientific Background: CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).
  
  Certificate of Analysis: 1ug/ml was sufficient for detection of 0.2ug CamKII by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: Synthetic peptide
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Dilution: 1u/ml was sufficient for detection of 0.2ug CAMKII by colorimetric immunoblot
• Storage Buffer: PBS pH7.4, 50% glycerol
• Reference: 1. Hughes K. et al. (2001) J. Biol. Chem. 276: 36008–36013. 2. Barria A. et al. (1997) Science 276: 2042–2045.
  3. Bennet M.K. and Kennedy M.B. (1987) Proc. Natl. Acad. Sci. U.S.A. 84: 1794-1798.
  4. Broke L., Srinivasan M. and Schulman H. (1995) J. Neurosci. 15: 6797-6808.
  5. Nghiem P., Saati S. M., Martens C. L., Gardner P. and Schulman H. (1993) J. Biol. Chem. 268: 5471-5479.
  6. Edman C.F. and Schulman H. (1994) Biochem. Biophys. Acta 1221: 90-102.
  7. Tombes R.M. and Krystal G.W., (1997) Biochem. Biophys. Acta 13555: 281-292.
  8. Means A.R. (2000) Mol. Endocrinol. 14: 4–12.
  9. Makhinson M. et al. (1999) J. Neurosci. 19: 2500–2510.
  10. Strack S. and Colbran R.J. (1998) J. Biol. Chem. 273: 20689-20692.
  11. Leonard S.A., Lim I.A., Hemsworth D.E., Horne M.C. and Hell J.W. (1999) Proc. Natl. Acad. Sci. U.S.A. 96: 3239-3244.
  12. Shen K. and Meyer Y. (1999) Science 284: 162-167.

Target Info

• Gene Symbol: Camk2a
• Gene Full Name: calcium/calmodulin-dependent protein kinase II alpha
• Alias Symbols: PKCCD, PK2CDD
• NCBI Gene Id: 25400
• Uniprot ID: P11275
• Protein Accession #: NP_037052.1