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CaMKII (Alpha-Specific) Antibody (OASE00036)

25ug
$149.00
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Gene Symbol:
Camk2a
NCBI Gene Id:
12322
Official Gene Full Name:
calcium/calmodulin-dependent protein kinase II alpha
Swissprot Id:
P11798
Protein Accession #:
NP_033922.1
Replacement Item:
This antibody may replace item sc-118982 from Santa Cruz Biotechnology.
Host:
Mouse
Clonality:
Monoclonal
Purification:
Protein G Purified
Application:
IF, IP, WB, IHC, RIA, ELISA
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express Camk2a.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express Camk2a.
Immunogen:
Partially purified rat CaMKII
Predicted Species Reactivity:
Mouse, Rat, Bovine
Reconstitution and Storage:
Store at -20C. Shipping in Blue Ice or 4C.
Concentration:
1mg/mL
Datasheets/Manuals:
Printable datasheet for OASE00036
Dilution:
0.1ug/mL was needed for detection of CaMKII in 20 ug of rat brain tissue
Clone:
6G9
Isotype:
IgG1
Additional Information:
Background Info: Detects ~50-60kDa protein, corresponding to the molecular mass of CaMKII alpha-subunit on SDS page immunoblots. Recognizes both phosphorylated and non-phosphorylated forms.
:::
Scientific Background: CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).
:::
Certificate of Analysis: 0.1ug/ml was sufficient for detection of CamKII in 20ug rat brain tissue extract by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.
Storage Buffer:
PBS pH7.4, 50% glycerol
Target Reference:
1. Hughes K. et al. (2001) J. Biol. Chem. 276: 36008–36013. 2. Barria A. et al. (1997) Science 276: 2042–2045.
3. Bennet M.K. and Kennedy M.B. (1987) Proc. Natl. Acad. Sci. U.S.A. 84: 1794-1798.
4. Broke L., Srinivasan M. and Schulman H. (1995) J. Neurosci. 15: 6797-6808.
5. Nghiem P., Saati S. M., Martens C. L., Gardner P. and Schulman H. (1993) J. Biol. Chem. 268: 5471-5479.
6. Edman C.F. and Schulman H. (1994) Biochem. Biophys. Acta 1221: 90-102.
7. Tombes R.M. and Krystal G.W., (1997) Biochem. Biophys. Acta 13555: 281-292.
8. Means A.R. (2000) Mol. Endocrinol. 14: 4–12.
9. Makhinson M. et al. (1999) J. Neurosci. 19: 2500–2510.
10. Strack S. and Colbran R.J. (1998) J. Biol. Chem. 273: 20689-20692.
11. Leonard S.A., Lim I.A., Hemsworth D.E., Horne M.C. and Hell J.W. (1999) Proc. Natl. Acad. Sci. U.S.A. 96: 3239-3244.
12. Shen K. and Meyer Y. (1999) Science 284: 162-167.

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