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Catalog No: OASE00037-HRP
Size:100ug
Price: $379.00
SKU
OASE00037-HRP
Availability: Domestic: within 3-5 days delivery | International: 3-5 days
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CaMKII (Alpha-Specific) Antibody : HRP (OASE00037-HRP)

Datasheets/ManualsPrintable datasheet for OASE00037-HRP
Product Info
Predicted Species ReactivityMouse, Rat, Bovine
Product FormatLiquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
ClonalityMonoclonal
Clone6G9
IsotypeIgG1
HostMouse
ConjugationHRP: Horseradish Peroxidase
ApplicationIF, IP, WB, IHC, RIA, ELISA
Additional InformationBackground Info: Detects ~50-60kDa protein, corresponding to the molecular mass of CaMKII alpha-subunit on SDS page immunoblots. Recognizes both phosphorylated and non-phosphorylated forms.
::Scientific Background: CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).
::Certificate of Analysis: 0.1ug/ml was sufficient for detection of CamKII in 20ug rat brain tissue extract by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.
Reconstitution and StorageAll conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
ImmunogenPartially purified rat CaMKII
PurificationProtein G Purified
Concentration0.6-0.7 mg/ml
Dilution0.1ug/mL was needed for detection of CaMKII in 20 ug of rat brain tissue
Storage BufferPBS pH7.4, 50% glycerol
Reference1. Hughes K. et al. (2001) J. Biol. Chem. 276: 36008–36013. 2. Barria A. et al. (1997) Science 276: 2042–2045.
3. Bennet M.K. and Kennedy M.B. (1987) Proc. Natl. Acad. Sci. U.S.A. 84: 1794-1798.
4. Broke L., Srinivasan M. and Schulman H. (1995) J. Neurosci. 15: 6797-6808.
5. Nghiem P., Saati S. M., Martens C. L., Gardner P. and Schulman H. (1993) J. Biol. Chem. 268: 5471-5479.
6. Edman C.F. and Schulman H. (1994) Biochem. Biophys. Acta 1221: 90-102.
7. Tombes R.M. and Krystal G.W., (1997) Biochem. Biophys. Acta 13555: 281-292.
8. Means A.R. (2000) Mol. Endocrinol. 14: 4–12.
9. Makhinson M. et al. (1999) J. Neurosci. 19: 2500–2510.
10. Strack S. and Colbran R.J. (1998) J. Biol. Chem. 273: 20689-20692.
11. Leonard S.A., Lim I.A., Hemsworth D.E., Horne M.C. and Hell J.W. (1999) Proc. Natl. Acad. Sci. U.S.A. 96: 3239-3244.
12. Shen K. and Meyer Y. (1999) Science 284: 162-167.
Gene SymbolCamk2a
Gene Full Namecalcium/calmodulin-dependent protein kinase II alpha
Alias SymbolsCaMKII, R74975, mKIAA0968
NCBI Gene Id12322
Uniprot IDP11798
Protein Accession #NP_033922.1
  1. What is the species homology for "CaMKII (Alpha-Specific) Antibody : HRP (OASE00037-HRP)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Mouse, Rat, Bovine".

  2. How long will it take to receive "CaMKII (Alpha-Specific) Antibody : HRP (OASE00037-HRP)"?

    This item is available "Domestic: within 3-5 days delivery | International: 3-5 days".

  3. What buffer format is "CaMKII (Alpha-Specific) Antibody : HRP (OASE00037-HRP)" provided in?

    This item is provided in "Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "CaMKII (Alpha-Specific) Antibody : HRP (OASE00037-HRP)"?

    This target may also be called "CaMKII, R74975, mKIAA0968" in publications.

  5. What is the shipping cost for "CaMKII (Alpha-Specific) Antibody : HRP (OASE00037-HRP)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "CaMKII (Alpha-Specific) Antibody : HRP (OASE00037-HRP)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "CaMKII (Alpha-Specific) Antibody : HRP (OASE00037-HRP)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "CaMKII (Alpha-Specific) Antibody : HRP (OASE00037-HRP)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "CAMK2A"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "CAMK2A"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "CAMK2A"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "CAMK2A"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "CAMK2A"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "CAMK2A"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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