CAMK2D Antibody (OAGA00902)

Catalog No: OAGA00902

Size:100 ul

CaMKII delta antibody detects CaMKII delta protein by western blot analysis.
A. 30 ?g 293T whole cell lysate/extract
B. 30 ?g A431 whole cell lysate/extract
C. 30 ?g HeLa whole cell lysate/extract
D. 30 ?g HepG2 whole cell lysate/extract
10 % SDS-PAGE
CaMKII delta antibody (OAGA00902) dilution: 1:1000

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
OAGA00902 diluted at 1:10000

Immunofluorescence analysis of paraformaldehyde-fixed A431, using CaMKII delta(OAGA00902) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rabbit
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, WB
• Additional Information: Background: The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a delta chain. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Distinct isoforms of this chain have different expression patterns.
  Positive Controls: 293T, A431, H1299, HeLa, HepG2, Raji, mouse brain, *rabbit pulmonary vein
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment contain a sequence corresponding to a region within amino acids 249 and 445 of CaMK2D (Uniprot ID#Q13557)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.97 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:1000-1:10000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: CAMK2D
• Gene Full Name: Calcium/calmodulin-dependent protein kinase type II subunit delta
• Alias Symbols: CAMKD
• NCBI Gene Id: 817
• Protein Name: Calcium/calmodulin-dependent protein kinase type II subunit delta
• Uniprot ID: Q13557
• Protein Accession #: NP_001212.2
• Molecular Weight: 54 kDa