CAMK2A ELISA Kit (Mouse) (OKEH06875)

Catalog No: OKEH06875

Size:96T

Mouse CAMK2A ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology Camk2a ELISA Kit (Mouse) (OKEH06875) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Camk2a antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Camk2a and incubated. The Camk2a found in the sample or standards competes with the biotinylated Camk2a for limited binding sites on the immobilized anti-Camk2a antibody. Excess unbound biotinylated Camk2a and sample or standard Camk2a is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Camk2a captured in the well and inversely proportional to the amount of Camk2a which was contained in the sample or standard.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into matrix = 96.6%
• ELISA Kit Reproducibility: Intra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates.
  Intra-Assay CV <7.5%
  Inter-Assay CV <9.6%
• ELISA Kit Component:
  • Camk2a Microplate: 96 Wells (12 x 8 Well strips)
  • Camk2a Lyophilized Standard: 2 x 50 ng
  • 100X Camk2a HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 0.39 ng/mL
• Specificity: Natural and recombinant Mouse Calcium/calmodulin-dependent protein kinase type II subunit alpha
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Camk2a
• Gene Full Name: calcium/calmodulin-dependent protein kinase II alpha
• Alias Symbols: alpha-CaMKII;calcium/calmodulin-dependent protein kinase type II subunit alpha;caM kinase II subunit alpha;CaMK II;CaMKII;caMK-II subunit alpha;mKIAA0968;R74975.
• NCBI Gene Id: 12322
• Protein Name: P11798
• Description of Target: Calcium/calmodulin-dependent protein kinase type II subunit alpha
• Uniprot ID: https://www.uniprot.org/uniprot/P11798
• Protein Accession #: NP_001273738
• Nucleotide Accession #: NM_001286809