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Catalog No: OKCD02321
Size:96WELLS
Price: $700.00
SKU
OKCD02321
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Datasheets/ManualsPrintable datasheet for CALU ELISA Kit (Rat) (OKCD02321)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calumenin (CALU). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calumenin (CALU). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calumenin (CALU), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Calumenin (CALU) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.781-50ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calumenin (CALU) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calumenin (CALU) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CALU Microplate96 Wells (12 x 8 Well strips)
CALU Lyophilized Standard2
100X Biotinylated CALU Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids
Sensitivity0.31 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Calumenin (CALU). No significant cross-reactivity or interference between Calumenin (CALU) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolCALU
Gene Full Namecalumenin
Alias SymbolsCBP-50;Crocalbin.
Protein NameCalumenin
Description of TargetInvolved in regulation of vitamin K-dependent carboxylation of multiple N-terminal glutamate residues. Seems to inhibit gamma-carboxylase GGCX. Binds 7 calcium ions with a low affinity (By similarity).
Uniprot IDO35783
Protein Accession #NP_001029070.1
Nucleotide Accession #NM_001033898.1
Protein Size (# AA)315
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