Calreticulin Antibody (OASE00365)

Catalog No: OASE00365 (Formerly GWB-6E7299)

Size:200ul

Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~35 kDa SOD (EC) protein using Rabbit Anti-SOD (EC) Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Human Cervical cancer cell line (HeLa) lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-SOD (EC) Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~35 kDa. Other Band(s): 50 kDa.

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-SOD (EC) Polyclonal Antibody. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-SOD (EC) Polyclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Golgi lumen. Exosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-SOD (EC) Antibody. (C) Composite.

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-SOD (EC) Polyclonal Antibody. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-SOD (EC) Polyclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Golgi lumen. Exosome. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-SOD (EC) Antibody. (C) Composite.

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Alpha B Crystallin Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Alpha B Crystallin Polyclonal Antibody at 1:120 for 12 hours at 4C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Actin filament bundles. Nuclear splicing speckles. Exosomes. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Alpha B Crystallin Antibody. (C) Composite. Heat Shocked at 42C for 1h.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Bovine, Dog, Chicken, Guinea Pig, Monkey, Pig, Hamster, Rabbit, Sheep
• Product Format: Liquid.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: WB, IHC, ICC, IF, IP, FC
• Additional Information: Cellular Location: Endoplasmic Reticulum, Cytoplasm, Cell Surface, Cytosol, Extracellular Matrix
• Reconstitution and Storage: Store at -20C. Shipping with Blue Ice or 4C.
• Immunogen: Human calreticulin synthetic peptide with a cysteine residue added and the peptide conjugated to KLH
• Purification: Peptide Affinity Purified
• Concentration: 1 mg/ml
• Specificity: Detects ~63kDa.
• Dilution: WB (1:1000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Application Info: WB- 1:5000
  A 1:5000 dilution was sufficient for detection of Calreticulin in 20ug of HeLa cell lysate by ECL immunoblot analysis.
  ICC/IF: 1:200
  Optimal dilutions for assays should be determined by the user.
• Reference: 1. Johnson S., et al. (2001) Trends Cell Biol 11: 122-129. 2. Smith M.J., et al. (1989) EMBO J. 8: 3581-3586.
  3. Ellgaard L., et al. (2001) Curr Opin Cell Biol. 13: 431-437.
  4. Krause K.H., and Michalak M. (1997) Cell. 88: 439-443.
  5. Nash P.D., et al. (1994) Mol Cell Biochem. 135: 71-78.
  6. Heal R., and McGivan J. (1998) Biochem J. 329: 389-394.
  7. Lucero H.A., et al. (1998) J Biol Chem. 273: 9857-9863.
  8. Tanaka S., et al. (2000) J Biol Chem. 275: 10388-10393.
  9. Yoon G.S., et al. (2000) Cancer Res. 60: 1117-1120.
  10. Antoniou A.N., et al. (2002) Immunology 106: 182-189.
  11. Wada I., et al. (1997) EMBO J. 16: 5420-5432.
  12. Laguerre D.B., et al. (1998) J Vir. 72: 4940-4949.
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Description: Calreticulin is a multifunctional, highly conserved Ca2+ -binding protein that is localized to the endoplasmic reticulum (ER), but has also been detected in the nucleus and nuclear envelop. Like many other ER proteins, it has the conserved ER retention KDEL (Lys-Asp-Glu-Leu) sequence at its C-terminus (1-3). CRT's three domains include a 180 residue N-terminal domain, a proline-rich P-domain (residues 189-288) that binds Ca2+ with high affinity and shares homology with calnexin (CNX) and calmegin, and a 110 residue C-terminal domain that binds Ca2+ with low affinity but high capacity (1,3). Recent studies suggest that this soluble ER protein has a multifunctional role. It appears to be involved in calcium storage and regulation as well as having a molecular chaperone activity. It has been shown to interact with the cytoskeleton and to be involved in the regulation of gene expression. Calreticulin may also play a role in cellular proliferation including its apparent activity in the proliferation of certain viruses within mammalian host cells (4, 5), and it has also been shown to be induced in response to various types of cell stress including amino acid deprivation (6). Close interconnections among protein synthesis, gene expression and calcium signaling have been observed by many researchers in recent years. Calreticulin might be centrally located and therefore it crucially participates in the coordination of many functions by the cell (4, 5). Studies also suggest its involvement in a few diseases such as systemic lupus erythematosus, rheumatoid arthritis, celiac disease, complete congenital heart block, and halothane hepatitis (1).

Target Info

• Gene Symbol: Calreticulin
• Gene Full Name: calreticulin
• Alias Symbols: CALR, Calregulin, cC1qR, CRP55, ERp60, HSCBP, RO, SSA, grp60
• NCBI Gene Id: 811
• Description of Target: Calreticulin is a multifunctional, highly conserved Ca2+ -binding protein that is localized to the endoplasmic reticulum (ER), but has also been detected in the nucleus and nuclear envelop. Like many other ER proteins, it has the conserved ER retention KDEL (Lys-Asp-Glu-Leu) sequence at its C-terminus (1-3). CRT's three domains include a 180 residue N-terminal domain, a proline-rich P-domain (residues 189-288) that binds Ca2+ with high affinity and shares homology with calnexin (CNX) and calmegin, and a 110 residue C-terminal domain that binds Ca2+ with low affinity but high capacity (1,3). Recent studies suggest that this soluble ER protein has a multifunctional role. It appears to be involved in calcium storage and regulation as well as having a molecular
  chaperone activity. It has been shown to interact with the cytoskeleton and to be involved in the regulation of gene expression. Calreticulin may also play a role in cellular proliferation including its apparent activity in the proliferation of certain viruses within mammalian host cells (4, 5), and it has also been shown to be induced in response to various types of cell stress including amino acid deprivation (6). Close interconnections among protein synthesis, gene expression and calcium signaling have been observed by many researchers in recent years. Calreticulin might be centrally located and therefore it crucially participates in the coordination of many functions by the cell (4, 5). Studies also suggest its involvement in a few diseases such as systemic lupus erythematosus, rheumatoid arthritis, celiac disease, complete congenital heart block, and halothane hepatitis (1).
  
• Uniprot ID: P27797
• Protein Accession #: NP_004334.1