Calreticulin Antibody : FITC (OASE00365-FITC)

Catalog No: OASE00365-FITC

Size:200ul

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Bovine, Canine, Chicken, Guinea Pig, Monkey, Pig, Hamster, Rabbit, Sheep
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: FITC: Fluorescein Isothiocyanate
• Application: WB, IHC, ICC, IP, IF
• Additional Information: Cellular Location: Endoplasmic Reticulum, Cytoplasm, Cell Surface, Cytosol, Extracellular Matrix
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: Human calreticulin synthetic peptide with a cysteine residue added and the peptide conjugated to KLH
• Purification: Rabbit antiserum
• Concentration: 1 mg/ml
• Specificity: Detects ~63kDa.
• Dilution: 1:5000-10000 (ECL) (WB)
• Application Info: WB- 1:5000
  A 1:5000 dilution was sufficient for detection of Calreticulin in 20ug of HeLa cell lysate by ECL immunoblot analysis.
  ICC/IF: 1:200
  Optimal dilutions for assays should be determined by the user.
• Reference: 1. Johnson S., et al. (2001) Trends Cell Biol 11: 122-129. 2. Smith M.J., et al. (1989) EMBO J. 8: 3581-3586.
  3. Ellgaard L., et al. (2001) Curr Opin Cell Biol. 13: 431-437.
  4. Krause K.H., and Michalak M. (1997) Cell. 88: 439-443.
  5. Nash P.D., et al. (1994) Mol Cell Biochem. 135: 71-78.
  6. Heal R., and McGivan J. (1998) Biochem J. 329: 389-394.
  7. Lucero H.A., et al. (1998) J Biol Chem. 273: 9857-9863.
  8. Tanaka S., et al. (2000) J Biol Chem. 275: 10388-10393.
  9. Yoon G.S., et al. (2000) Cancer Res. 60: 1117-1120.
  10. Antoniou A.N., et al. (2002) Immunology 106: 182-189.
  11. Wada I., et al. (1997) EMBO J. 16: 5420-5432.
  12. Laguerre D.B., et al. (1998) J Vir. 72: 4940-4949.
• Storage Buffer: Rabbit antiserum

Target Info

• Gene Symbol: CALR
• Gene Full Name: calreticulin
• Alias Symbols: RO, CRT, SSA, cC1qR, HEL-S-99n
• NCBI Gene Id: 811
• Description of Target: Calreticulin is a multifunctional, highly conserved Ca2+ -binding protein that is localized to the endoplasmic reticulum (ER), but has also been detected in the nucleus and nuclear envelop. Like many other ER proteins, it has the conserved ER retention KDEL (Lys-Asp-Glu-Leu) sequence at its C-terminus (1-3). CRT's three domains include a 180 residue N-terminal domain, a proline-rich P-domain (residues 189-288) that binds Ca2+ with high affinity and shares homology with calnexin (CNX) and calmegin, and a 110 residue C-terminal domain that binds Ca2+ with low affinity but high capacity (1,3). Recent studies suggest that this soluble ER protein has a multifunctional role. It appears to be involved in calcium storage and regulation as well as having a molecular
  chaperone activity. It has been shown to interact with the cytoskeleton and to be involved in the regulation of gene expression. Calreticulin may also play a role in cellular proliferation including its apparent activity in the proliferation of certain viruses within mammalian host cells (4, 5), and it has also been shown to be induced in response to various types of cell stress including amino acid deprivation (6). Close interconnections among protein synthesis, gene expression and calcium signaling have been observed by many researchers in recent years. Calreticulin might be centrally located and therefore it crucially participates in the coordination of many functions by the cell (4, 5). Studies also suggest its involvement in a few diseases such as systemic lupus erythematosus, rheumatoid arthritis, celiac disease, complete congenital heart block, and halothane hepatitis (1).
  
• Uniprot ID: P27797
• Protein Accession #: NP_004334.1