CALR Recombinant Protein (Human) (OPED00014)

Catalog No: OPED00014

Size:50 ug

SDS-PAGE Analysis of Calreticulin (human), (recombinant) (His-tag). Lane 1: MW Marker; Lane 2: 1ug Calreticulin.

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Product Format: Liquid, In 50mM TRIS, pH 7.5, containing 150mM sodium chloride, 5mM calcium chloride and 5% glycerol.
• Host: E. Coli
• Application: WB
• Additional Information: The multifunctional, multi-compartmental protein Calreticulin (Crt) functions as a soluble molecular chaperone of new or misfolded proteins as well as a calcium-binding protein. Most abundant in the ER lumen, Crt expression also occurs in other membrane-bound organelles, the cell surface, and extracellularly. Also known as CRP-55, calregulin and HACBP (high affinity calcium-binding protein). Crt contains the ER retrieval sequence, KDEL, and is the soluble paralog of the ER membrane protein Calnexin (Cnx). Crt’s three domains include a 180 residue N-terminal domain, a proline-rich P-domain (residues 189-288) that binds calcium with high affinity and shares homology with Cnx and calmegin, and a 110 residue C-terminal domain that binds calcium with low affinity but high capacity. The P-domain may interact with the co-chaperone ERp57 (Grp58), a thiol reductase. The NMR structure of the P-domain consists of an extended hairpin that appears to form a curved protrusion from the Crt core domain. Both Crt and its membrane bound homolog CNX interact with proteins and glycoproteins possessing monoglucosylated N-glycans. The Crt/Cnx cycle promotes correct folding, inhibits aggregation of folding intermediates, blocks premature oligomerization, regulates ER degradation, and prevents incompletely folded glycoproteins from exiting to the Golgi complex. Crt also appears to function as an auto-antigen in systemic lupus erythematosus, rheumatoid arthritis, celiac disease, complete congenital heart block, and halothane hepatitis. A diversity of additional functions attributed to Crt includes adhesion, blood function, and cardiac and neuronal development gene expression.
• Reconstitution and Storage: Store at -80C
• Purification: Purified by multi-step chromatography.
• Purity: >90% (SDS-PAGE; Western blot)
• Application Info: Western blot control.

Target Info

• Gene Symbol: CALR
• Gene Full Name: calreticulin
• Alias Symbols: calregulin;calreticulin;cC1qR;CRP55;CRT;endoplasmic reticulum resident protein 60;epididymis secretory sperm binding protein Li 99n;ERp60;grp60;HACBP;HEL-S-99n;RO;Sicca syndrome antigen A (autoantigen Ro; calreticulin);SSA.
• NCBI Gene Id: 811
• Protein Name: Calreticulin
• Description of Target: Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis (By similarity).
• Uniprot ID: P27797
• Protein Accession #: NP_001241.1
• Nucleotide Accession #: NM_001250.5
• Protein Size (# AA): Recombinant
• Molecular Weight: 59 kDa