CALR ELISA Kit (Dog) (OKEH08056)

Catalog No: OKEH08056

Size:96T

Product Info

• Predicted Species Reactivity: Canis lupus, Dog
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	116-128% (n = 20)	103-115% (n = 20)	86-98% (n = 20)	102-111% (n = 20)
  EDTA plasma(n=5)	102-113% (n = 20)	98-111% (n = 20)	93-103% (n = 20)	98-106% (n = 20)
  heparin plasma(n=5)	107-120% (n = 20)	92-104% (n = 20)	110-119% (n = 20)	110-120% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology CALR ELISA Kit (Dog) (OKEH08056) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CALR has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CALR is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CALR captured in well.
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 104% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=7.2% (n = 20)
  Mean Inter-assay CV%: <=9.3% (n = 20)
• ELISA Kit Component:
  • CALR Microplate: 96 Wells (12 x 8 Well strips)
  • CALR Lyophilized Standard: 2
  • 100X Biotinylated CALR Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export (By similarity). Plays a major role in Ca2(+) storage in pancreatic cells.
  Subcellular Location: Endoplasmic reticulum lumen
• Reconstitution and Storage: Store as indicated in product manual.
• Specificity: Natural and recombinant Dog Calreticulin
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CALR
• Alias Symbols: Calregulin;CRP55;Endoplasmic reticulum resident protein 60;HACBP.
• Protein Name: P28490
• Description of Target: Calreticulin
• Uniprot ID: https://www.uniprot.org/uniprot/P28490