CALR Chemi-Luminescent ELISA Kit (Rat) (OKCD05568)

Catalog No: OKCD05568

Size:96WELLS

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Calreticulin (CALR). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Calreticulin (CALR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Calreticulin (CALR) level in the sample or standard.
• ELISA Kit Range: 2.74-2,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calreticulin (CALR) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calreticulin (CALR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CALR Microplate: 96 Wells (12 x 8 Well strips)
  • CALR Lyophilized Standard: 2
  • 100X CALR HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 1.21pg/mL

Target Info

• Gene Symbol: Calr
• Gene Full Name: calreticulin
• Alias Symbols: CABP3;CALBP;calcium-binding protein 3;calregulin;calreticulin;CRP55;endoplasmic reticulum resident protein 60;ERp60;HACBP.
• NCBI Gene Id: 64202
• Protein Name: Calreticulin
• Description of Target: Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis.
• Uniprot ID: P18418
• Protein Accession #: NP_071794.1
• Nucleotide Accession #: NM_022399.2