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Catalog No: OKEH02542 (Formerly GWB-885R52)
Price: $700.00
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKEH02542
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Calcitriol ELISA Kit (OKEH02542) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Calcitriol antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Calcitriol and incubated. The Calcitriol found in the sample or standards competes with the biotinylated Calcitriol for limited binding sites on the immobilized anti-Calcitriol antibody. Excess unbound biotinylated Calcitriol and sample or standard Calcitriol is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Calcitriol captured in the well and inversely proportional to the amount of Calcitriol which was contained in the sample or standard.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.8% (n = 20)
Mean Inter-assay CV%: < 10.1% (n = 20)
ELISA Kit Component
Anti-Calcitriol Microplate96 Wells (12 x 8 Well strips)
Calcitriol Lyophilized Standard2 x 1,000 pg
Sample Diluent1 x 20 mL
100X Calcitriol-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: Calcitriol
Target Name: Calcitriol
::Pubchem: 5280453
::Chemical formula: C27H44O3
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity3.6 pg/mL
SpecificityNatural and recombinant General Calcitriol

Temperature-dependent vitamin D signaling regulates developmental trajectory associated with diapause in an annual killifish. Proc Natl Acad Sci U S A. 115, 12763-12768 (2018). 30446615

Assay InfoAssay Methodology: Quantitative Competitive ELISA
Alias Symbols1,25-dihydroxycholecalciferol;1,25-dihydroxyvitamin D3
Protein NameCalcitriol
Description of TargetCalcitriol, also called 1,25-dihydroxycholecalciferol or 1,25-dihydroxyvitamin D3, is the hormonally active form of vitamin D with three hydroxyl groups (abbreviated 1,25-(OH)2D3 or simply 1,25(OH)2D). It increases the level of calcium (Ca2+) in the blood by (1) increasing the uptake of calcium from the gut into the blood, (2) decreasing the transfer of calcium from blood to the urine by the kidney, and (3) increasing the release of calcium into the blood from bone. Calcitriol is produced in the cells of the proximal tubule of the nephron in the kidneys by the action of 25-hydroxyvitamin D3 1-alpha-hydroxylase, a mitochondrial oxygenase and an enzyme which catalyzes the hydroxylation of 25-hydroxycholecalciferol (calcifediol). The activity of the enzyme is stimulated by PTH. The reaction is an important control point in Ca2+ homeostasis. The production of calcitriol is also increased by prolactin, a hormone which stimulates lactogenesis (the formation of breast milk), a process which requires large amounts of calcium. It is decreased by high levels of serum phosphate and by an increase in the production of the hormone FGF-23 by osteocyte cells in bone.
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