CALCA ELISA Kit (Pig) (OKCA02484)

Catalog No: OKCA02484

Size:96 Wells

Pig CALCA ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Pig
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  Neat	93	90-99
  1:2	95	85-100
  1:4	94	89-103
  1:8	95	86-102

• ELISA Kit Principle: Aviva Systems Biology CALCA ELISA Kit (Pig) (OKCA02484) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with CALCA. Sample or standards are added to the wells along with a fixed quantity of HRP-Conjugated CALCA Detector Antibody and incubated. The CALCA found in the sample or standards competes with immobilized CALCA for binding with the HRP-Conjugated CALCA Detector Antibody. Wells are washed and an enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product. Addition of acidic stop solution changes the coloration to yellow. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated CALCAcaptured in the well and inversely proportional to the amount of CALCA which was contained in the sample or standard.
• ELISA Kit Range: 31.25 - 500 pg/mL
• ELISA Kit Recovery:

  Sample Type	Mean Recovery (%)	Range (%)
  Serum (n=5)	95	90-100
  EDTA Plasma (n=4)	92	85-101

• ELISA Kit Reproducibility: Mean Intra-Assay: CV <12% (n=20)
  Mean Inter-Assay: CV <15% (n=24)
• ELISA Kit Component:
  • CALCA Microplate: 96 Wells (12 x 8 Well strips)
  • CALCA Lyophilized Standard: 5 x 1 mL
  • 100X HRP-Conjugated CALCA Detector Antibody: 1 x 6 mL
  • HRP-Conjugate Diluent: 1 x 6 mL
  • 20X Wash Buffer: 1 x 15 mL
  • Substrate A: 1 x 7 mL
  • Substrate B: 1 x 7 mL
  • Stop Solution: 1 x 7 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 15.6 pg/mL
• Assay Info: Assay Methodology: Quantitative Competitive Inhibition Immunoassay

Target Info

• Gene Symbol: CALCA
• Alias Symbols: CALC1, CGRP, CGRP-I, CGRP1, CT, KC, MGC126648, calcitonin 1, calcitonin gene-related peptide 1, calcitonin/calcitonin-related polypeptide, alpha, katacalcin
• Protein Name: Calcitonin gene-related peptide
• Description of Target: CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role.
• Uniprot ID: P30880