Size:96 Wells
$469.00
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SKU
OKEH05611
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CALCA ELISA Kit (Mouse) (OKEH05611)

Catalog#: OKEH05611
Domestic: within 1-2 weeks delivery | International: 1-2 weeks
More Information
Predicted Species Reactivity Mouse
Kit Detection Method Colorimetric, OD450 nm
Kit Duration ~3 Hours
Kit Principle Aviva Systems Biology Calca ELISA Kit (Mouse) (OKEH05611) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Calca has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Calca is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Calca captured in the well.
Kit Range 31.2 - 2000pg/mL
Kit Recovery Mean recovery when spiking into Serum and Plasma = 105.5%
Kit Reproducibility Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - <4.6%, n = 20
Mean Inter-Assay Precision - <7.8%, n = 20
Kit Component
ComponentAmount
CALCA Microplate96 Wells (12 x 8 Well strips)
CALCA Lyophilized Standard2
100X Biotinylated CALCA Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and Storage Store as indicated in product manual.
Sample Type Serum, plasma and other biological fluids
Sensitivity 15.78 pg/mL
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity Natural and recombinant Mouse Calcitonin gene-related peptide 1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Gene Symbol CALCA
Official Gene Full Name Calcitonin/calcitonin-related polypeptide, alpha
Alias Symbols Alpha CGRP, Alpha-type CGRP, CA, Calc, Calc1, calcitonin, Calcitonin, Calcitonin gene-related peptide 1, Calcitonin gene-related peptide I, Cgrp, CGRP1, CGRP-1, CGRP-I, Ct, CT, Ctn
NCBI Gene Id 12310
Protein Name Calcitonin gene-related peptide 1
Description of Target CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role. It also elevates platelet cAMP.
Swissprot Id Q99JA0
Protein Accession # NP_001029126.1
Nucleotide Accession # NM_001033954.3
Protein Size (# AA) 128
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express CALCA.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express CALCA.
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