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Catalog No: OKCD00726 (Formerly GWB-KBBNN2)
Size:96WELLS
Price: $625.00
SKU
OKCD00726
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Datasheets/ManualsPrintable datasheet for CALCA ELISA Kit (Mouse) (OKCD00726)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)98-105%98-105%78-97%83-99%
EDTA plasma(n=5)87-102%80-102%89-101%78-92%
heparin plasma(n=5)93-105%87-94%91-98%84-95%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Procalcitonin (PCT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Procalcitonin (PCT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Procalcitonin (PCT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Procalcitonin (PCT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)80-9583
EDTA plasma(n=5)81-9087
heparin plasma(n=5)89-9792
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Procalcitonin (PCT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Procalcitonin (PCT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Calca Microplate96 Wells (12 x 8 Well Strips)
Calca Lyophilized Standard2 x 100 pg
100X Biotinylated Calca Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity< 0.64 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Procalcitonin (PCT). No significant cross-reactivity or interference between Procalcitonin (PCT) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Description
Gene SymbolCalca
Gene Full Namecalcitonin/calcitonin-related polypeptide, alpha
Alias Symbolsalpha-type CGRP;C;CA;Calc;Calc1;calcitonin gene-related peptide 1;calcitonin gene-related peptide I;Cg;Cgrp;CGRP1;CGRP-1;Ct;Ctn.
NCBI Gene Id12310
Protein NameCalcitonin
Description of TargetCauses a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones.
Uniprot IDP70160
Protein Accession #NP_001292545
Nucleotide Accession #NM_001305616.1
Protein Size (# AA)136
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