Catalog No: OKCD00726 (Formerly GWB-KBBNN2)
Size:96 Wells
Price: $625.00
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
EDTA plasma(n=5)87-102%80-102%89-101%78-92%
heparin plasma(n=5)93-105%87-94%91-98%84-95%
ELISA Kit PrincipleAviva Systems Biology Calca ELISA Kit (Mouse) (OKCD00726) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Calca has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Calca is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Calcacaptured in well.
ELISA Kit Range1.56-100 pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
EDTA plasma(n=5)81-9087
heparin plasma(n=5)89-9792
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level Calca were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level Calca were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
ELISA Kit Component
Anti-Calca Microplate96 Wells (12 x 8 Well Strips)
Calca Lyophilized Standard2 x 100 pg
100X Biotinylated Calca Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity< 0.64 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Procalcitonin (PCT). No significant cross-reactivity or interference between Procalcitonin (PCT) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCALCA
Gene Full Namecalcitonin/calcitonin-related polypeptide, alpha
Alias SymbolsC, CA, Cg, Ct, Ctn, Calc, Cgrp, CGRP1, Calc1, CGRP-1
NCBI Gene Id12310
Protein Namecalcitonin gene-related peptide 1
Description of TargetCauses a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones.
Uniprot IDP70160
Protein Accession #NP_001292545
Nucleotide Accession #NM_001305616.1
Protein Size (# AA)136
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