CALCA ELISA Kit (Mouse) (OKCD00726)

Catalog No: OKCD00726 (Formerly GWB-KBBNN2)

Size:96 Wells

Mouse Calca ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	98-105%	98-105%	78-97%	83-99%
  EDTA plasma(n=5)	87-102%	80-102%	89-101%	78-92%
  heparin plasma(n=5)	93-105%	87-94%	91-98%	84-95%

• ELISA Kit Principle: Aviva Systems Biology Calca ELISA Kit (Mouse) (OKCD00726) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Calca has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Calca is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Calcacaptured in well.
• ELISA Kit Range: 1.56-100 pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	80-95	83
  EDTA plasma(n=5)	81-90	87
  heparin plasma(n=5)	89-97	92

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level Calca were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level Calca were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <10%
  Inter-Assay CV: <12%
• ELISA Kit Component:
  • Anti-Calca Microplate: 96 Wells (12 x 8 Well Strips)
  • Calca Lyophilized Standard: 2 x 100 pg
  • 100X Biotinylated Calca Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 0.64 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Procalcitonin (PCT). No significant cross-reactivity or interference between Procalcitonin (PCT) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: CALCA
• Gene Full Name: calcitonin/calcitonin-related polypeptide, alpha
• Alias Symbols: C, CA, Cg, Ct, Ctn, Calc, Cgrp, CGRP1, Calc1, CGRP-1
• NCBI Gene Id: 12310
• Protein Name: calcitonin gene-related peptide 1
• Description of Target: Causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones.
• Uniprot ID: P70160
• Protein Accession #: NP_001292545
• Nucleotide Accession #: NM_001305616.1
• Protein Size (# AA): 136