CALCA ELISA Kit (Mouse) (OKCD00726)

Catalog No: OKCD00726 (Formerly GWB-KBBNN2)

Size:96WELLS

Mouse Calca ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: ELISA-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	98-105%	98-105%	78-97%	83-99%
  EDTA plasma(n=5)	87-102%	80-102%	89-101%	78-92%
  heparin plasma(n=5)	93-105%	87-94%	91-98%	84-95%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Procalcitonin (PCT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Procalcitonin (PCT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Procalcitonin (PCT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Procalcitonin (PCT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	80-95	83
  EDTA plasma(n=5)	81-90	87
  heparin plasma(n=5)	89-97	92

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Procalcitonin (PCT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Procalcitonin (PCT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Calca Microplate: 96 Wells (12 x 8 Well Strips)
  • Calca Lyophilized Standard: 2 x 100 pg
  • 100X Biotinylated Calca Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 0.64 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Procalcitonin (PCT). No significant cross-reactivity or interference between Procalcitonin (PCT) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Calca
• Gene Full Name: calcitonin/calcitonin-related polypeptide, alpha
• Alias Symbols: alpha-type CGRP;C;CA;Calc;Calc1;calcitonin gene-related peptide 1;calcitonin gene-related peptide I;Cg;Cgrp;CGRP1;CGRP-1;Ct;Ctn.
• NCBI Gene Id: 12310
• Protein Name: Calcitonin
• Description of Target: Causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones.
• Uniprot ID: P70160
• Protein Accession #: NP_001292545
• Nucleotide Accession #: NM_001305616.1
• Protein Size (# AA): 136