CALCA Antibody (OAPC00089)

Catalog No: OAPC00089

Size:100 ul

Immunostaining of CGRP (red) in cryopreserved DRG neurons grown on coverslips. 40xmag. Green staining is PGP9.5. Yellow staining is the colocalization of CGRP and PGP9.5. Cells were obtained photo was provided courtesy of QBMCellScience.

Product Info

• Predicted Species Reactivity: Human, Rat
• Product Format: Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Clonality: Monoclonal
• Isotype: IgG^2a
• Host: Mouse
• Application: ICC
• Additional Information: Packaging :
  100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Reconstitution and Storage: Store at -20C in undiluted aliquots; stable for at least one year after date of receipt. Avoid freeze/thaw cycles.
• Immunogen: Rat alpha-CGRP
• Purification: Protein G purified
• Predicted Homology Based on Immunogen Sequence: Human; Rat
• Specificity: Specific for Calcitonin Gene Related Peptide. Also recognizes rat beta-CGRP and human alpha-CGRP (10-fold less sensitivity compared to rat alpha-CGRP). Suggested positive controls are dorsal root ganglia, pancreas and gut.
• Application Info: The antibody has been directly tested for reactivity in rat and human. Other species have not yet been tested. It is expected that the antibody will react with most mammals.
  This antibody is an excellent tool for immunohistochemistry and for multiple labeling immunofluorescence of peripheral tissue like stomach, intestine, pancreas, sensory ganglia, and all the regions of the body that are innervated by primary afferent neurons as well as central nervous tissue.
  Immunostaining of CGRP (red) in cryopreserved DRG neurons grown on coverslips. 40xmag. Green staining is PGP9.5. Yellow staining is the colocalization of CGRP and PGP9.5. Cells were obtained photo was provided courtesy of QBMCellScience.
  
• Reference: Brain SD, Williams TJ, Tippins JR, Morris HR, MacIntyre I (1985) Calcitonin gene related peptide is a potent vasodilator. Nature 313 (5997): 54-6
  Edvinsson L (2003) New therapeutic target in primary headaches—blocking the CGRP receptor. Expert Opin Ther Targets.7(3):377-83
  Wong HC, Tache' Y, Lloud KCK, Yang H, Sternini C, Holzer P, Walsh JH (1993) Monoclonal antibody to rat alpha-CGRP: production, charactrization, and in vivo immunoneutralization activity. Hybridoma. 12:93-106.

Target Info

• Gene Symbol: CALCA
• Alias Symbols: CAL6, CGRP, Cal1, Calc, RATCAL6, calcitonin
• NCBI Gene Id: 24241
• Protein Name: Calcitonin gene-related peptide 1
• Description of Target: Calcitonin Gene Related Peptide [CGRP] is a 37 amino acid peptide and is the most potent endogenous vasodilator currently known (Brain et al.,1985). CGRP is primarily produced in nervous tissue, however, its receptors are expressed throughout the body. CGRP is also strongly implicated in the vasodilatory effect of endogenous cannabinoid anandamide in the brain. This effect was found to be antagonized by capsazepine. CGRP is also currently a major target of research in regards to factors effecting the onset of migraine headaches (Edvinsson L., 2003). CGRP antibodies have been used successfully in immunoneutralization (Wong et al., 1993)
• Uniprot ID: P01256
• Protein Accession #: NP_001029127.1