CALB2 Protein (OPPA01313)

Catalog No: OPPA01313 (Formerly GWB-1DAE98)

Size:5UG

Product Info

• Predicted Species Reactivity: Human
• Product Format: The protein was lyophilized from a concentrated solution (1mg/ml) containing 10mM Tris pH-7.4, 50mM sodium chloride and 1mM b-mercaptoethanol. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Host: E. Coli
• Application: WB, IHC, ELISA
• Additional Information: Solubility: It is recommended to reconstitute the lyophilized CABP29K in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
  Product Description: Calretinin/CR/CLB2/CALB2/Calbindin D29K protein (mouse/rat/human 271aa, chromosome 16q22.1, mol wt ~29 kDa) also belongs to the Calbindin family. It is most closely related to CABP28K (~55% identity). It may be alternatively spliced to a C-terminally truncated fragment, Calretinin-22K in some tumor cell lines. Calretinin is highly expressed in the cerebellum, olfactory bulb, and in auditory neurons. Calretinin gene inactivation in mice eliminated long-term potentiation induction in the gentate gyrus and impaired motor coordination.Recombinant Human Calbindin 29kDa is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.
• Reconstitution and Storage: Lyophilized CABP29K although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution CABP29K should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles.
• Purity: Greater than 90% as determined by SDS-PAGE.
• Application Info: This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection.For adsorption we suggest the following procedureA- Dilute 1 ul of the antiserum against calretinin in 5 ml of the usual buffer for immunohistochemistry (final dilution 1:5'000).B- Add 1 ug of recombinant protein to 1ml of the diluted antibody solution and mix well.C- Incubate for at least 6 hours in the cold.D- Apply to tissue-sections and incubate for 3 days.E - Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB).As a result, the immunostaining should be strongly reduced or even completely prevented.

Target Info

• Gene Symbol: CaBP29K
• Alias Symbols: CR, CAL2, CAB29
• NCBI Gene Id: 794
• Protein Name: Calretinin
• Description of Target: Recombinant Human Calbindin D-29K
• Uniprot ID: P22676
• Protein Accession #: NP_001731.2
• Protein Size (# AA): Recombinant