Catalog No: OKEH00768
Size:96T
Price: $525.00
SKU
OKEH00768
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CALB1 ELISA Kit (Mouse) (OKEH00768) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CALB1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CALB1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CALB1 captured in well.
ELISA Kit Range31.2-2000pg/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 7.3% (n = 20)
Mean Inter-assay CV%: < 9.2% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-CALB1 Microplate96 Wells (12 x 8 Well strips)
CALB1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated CALB1 Detector Antibody1 x 120 ul
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity15.6 pg/mL
SpecificityNatural and recombinant Mouse Calbindin
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Description
Gene SymbolCalb1
Gene Full Namecalbindin 1
Alias SymbolsBra;Brain-2;Ca;cal;Calb;Calb-1;calbindin;calbindin 1, 28kDa;calbindin D28;calbindin-28K;calbindin-D (28k);CalbindinD28K;CB;D-28K;PCD-29;spot 35 protein;vitamin D-dependent calcium-binding protein, avian-type.
NCBI Gene Id12307
Protein NameP12658
Description of TargetCalbindin
Uniprot IDhttps://www.uniprot.org/uniprot/P12658
Protein Accession #NP_033918.1
Nucleotide Accession #NM_009788.4
Protein Size (# AA)261
Molecular Weight29 kDa
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