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| Datasheets/Manuals | Printable datasheet for CALB1 Antibody (OABB01514) |
|---|
| Tested Species Reactivity | Human, Mouse, Rat |
|---|---|
| Predicted Species Reactivity | Bovine|Horse|Human|Mouse|Rat |
| Product Format | Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
| Clonality | Polyclonal |
| Clone | Polyclonal |
| Isotype | Rabbit IgG |
| Host | Rabbit |
| Application | Flow cytometry|Immunocytochemistry|Immunohistochemistry|Western blot |
| Additional Information | Notes: WB: The detection limit for Calbindin is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. |
| :: | Background: Calbindin is a calcium-binding protein belonging to the troponin C superfamily. Calretinin is expressed in central and peripheral nervous system and in many normal and pathological tissues. The rat and human calretinin exhibit 98% sequence homology and 91% homology to many other species. Two calcium binding proteins, calbindin and calretinin, have been reported to be expressed in abundance in Purkinje cells and other cell types in the cerebellum. |
| Reconstitution and Storage | 2°C to 8°C|-20°C |
| Immunogen | E.coli-derived human Calbindin recombinant protein (Position: A2-E175). Human Calbindin shares 99% amino acid (aa) sequence identity with both mouse and rat Calbindin. |
| Purification | Affinity Purified |
| Concentration | 500 ug/ml |
| Specificity | No cross reactivity with other proteins. |
| Application Info | Western blot: 0.1-0.5 ug/ml: Rat: Human Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human, Mouse, Rat: By Heat Immunohistochemistry (Frozen Section): 0.5-1 ug/ml: Human Immunocytochemistry: 0.5-1 ug/ml: Human Flow Cytometry: 1-3 ug/1x10^6 cells: Human |
| Storage Buffer | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
| Description | Rabbit IgG polyclonal antibody for Calbindin(CALB1) detection. Tested with WB, IHC-P, IHC-F, ICC, FCM in Human;Mouse;Rat. |
| Gene Symbol | CALB1 |
|---|---|
| Gene Full Name | calbindin 1 |
| Alias Symbols | CALB;calbindin;calbindin 1, (28kD);calbindin 1, 28kDa;calbindin 27;calbindin D28;D-28K;RTVL-H protein;vitamin D-dependent calcium-binding protein, avian-type. |
| NCBI Gene Id | 793 |
| Protein Name | Calbindin |
| Description of Target | Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase. |
| Uniprot ID | P05937 |
| Molecular Weight | 30025 MW |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "CALB1 Antibody (OABB01514)"?
The tested species reactivity for this item is "Human, Mouse, Rat". This antibody is predicted to have homology to "Bovine|Horse|Human|Mouse|Rat".
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How long will it take to receive "CALB1 Antibody (OABB01514)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "CALB1 Antibody (OABB01514)" provided in?
This item is provided in "Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "CALB1 Antibody (OABB01514)"?
This target may also be called "CALB;calbindin;calbindin 1, (28kD);calbindin 1, 28kDa;calbindin 27;calbindin D28;D-28K;RTVL-H protein;vitamin D-dependent calcium-binding protein, avian-type." in publications.
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What is the shipping cost for "CALB1 Antibody (OABB01514)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "CALB1 Antibody (OABB01514)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "CALB1 Antibody (OABB01514)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "30025 MW".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "CALB1 Antibody (OABB01514)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "CALB1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "CALB1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "CALB1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "CALB1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "CALB1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "CALB1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
