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Catalog No: OKBB00416
Size:96 Wells
Price: $583.00
SKU
OKBB00416
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva's human Cadherin-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Cadherin-2 has been precoated onto 96-well plates. Standards(NS0, D160-A724) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cadherin-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Cadherin-2 amount of sample captured in plate.
ELISA Kit Range156 pg/ml - 10,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n 161616242424
Mean (pg/mL)9.622.438.212.526.440.7
SD0.3650.9631.9860.6751.612.768
CV%3.84.35.25.46.16.8
ELISA Kit Component
ComponentAmount
96-Well plate Pre-coated with Anti-Human N-Cadherin Antibody1 x (12 x 8 Well Strip)
Lyophilized Recombinant Human N-Cadherin Standard2 x 50 ng/tube
100X Biotinylated Anti-Human N-Cadherin Antibody1 x 130 uL
100X Avidin-Biotin-Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample TypeCell culture supernatants, serum and plasma (heparin, EDTA)
Sensitivity<20 pg/ml
SpecificityNatural and recombinant human Cadherin-2
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
ImmunogenExpression system for standard: NS0; Immunogen sequence: D160-A724
DescriptionFor quantitative detection of human Cadherin-2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Gene SymbolCDH2
Gene Full Namecadherin 2, type 1, N-cadherin (neuronal)
Alias SymbolsCDw325, CDHN, Neural cadherin, N-cadherin, CD325
NCBI Gene Id1000
Protein NameCadherin-2
Description of TargetCadherin-2 (CDH2), also known as neural cadherin (NCAD), is a protein that in humans is encoded by the CDH2 gene. It is a classical cadherin from the cadherin superfamily. This gene is mapped to 18q12.1. Cadherin-2 is expressed in the brain, skeletal and cardiac muscle. Cadherin-2 is commonly found in cancer cells and provides a mechanism for transendothelial migration. It is a calcium dependent cell-cell adhesion glycoprotein comprising five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.
Uniprot IDP19022
Protein Accession #NP_001295105.1
Nucleotide Accession #NM_001308176.1
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