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Datasheets/Manuals | Printable datasheet for CAD Antibody (OAPB00038) (OAPB00038) |
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Predicted Species Reactivity | Human|Mouse|Rat |
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Product Format | Liquid. CAD Antibody is supplied in PBS containing 0.02% sodium azide. |
Clonality | Polyclonal |
Host | Rabbit |
Conjugation | Unconjugated |
Application | Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot |
:: | Buffer: Antibody is supplied in PBS containing 0.02% sodium azide. |
Reconstitution and Storage | CAD antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Immunogen | CAD antibody was raised against a 16 amino acid peptide near the carboxy terminus of murine CAD. The immunogen is located within the last 50 amino acids of CAD. |
Purification | Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Mouse |
Concentration | 1 mg/ml |
Blocking Peptide | For anti-CAD (OAPB00038) antibody is Catalog # OEPB02166 |
Application Info | CAD antibody antibody can be used for detection of CAD by Western blot at 1:500 to 1:1000 dilution. A 40 kDa band should be detected. (Optimal dilution should be determined by user) |
Reference | Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50 ;Sakahira H, Enari M, Nagata S. Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis. Nature 1998;391:96-99 ;Liu X, Li P, Widlak P, Zou H, Luo X, Garrard WT, Wang X The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis. Proc Natl Acad Sci USA 1998;95:8461-6 ;Halenbeck R, MacDonald H, Roulston A, Chen TT, Conroy L, Williams LT. CPAN, a human nuclease regulated by the caspase-sensitive inhibitor DFF45. Curr Biol 1998;8:537-40 |
Gene Symbol | Dffb |
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Gene Full Name | DNA fragmentation factor, beta subunit |
Alias Symbols | 40kD;40kDa;5730477D02Rik;C;CA;CAD;caspase-activated deoxyribonuclease;caspase-activated DNase;CPAN;DFF40;DFF-40;Didf;Didff;DNA fragmentation factor 40 kDa subunit;DNA fragmentation factor subunit beta;DNA fragmentation factor, 40 kD, beta subunit;DNase inhibited by DNA fragmentation factor. |
NCBI Gene Id | 13368 |
Protein Name | DNA fragmentation factor subunit beta |
Description of Target | Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology. |
Uniprot ID | O54788 |
Protein Accession # | NP_004332.2 |
Nucleotide Accession # | NM_004341.4 |
Molecular Weight | 40 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "CAD Antibody (OAPB00038)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "CAD Antibody (OAPB00038)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "CAD Antibody (OAPB00038)" provided in?
This item is provided in "Liquid. CAD Antibody is supplied in PBS containing 0.02% sodium azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "CAD Antibody (OAPB00038)"?
This target may also be called "40kD;40kDa;5730477D02Rik;C;CA;CAD;caspase-activated deoxyribonuclease;caspase-activated DNase;CPAN;DFF40;DFF-40;Didf;Didff;DNA fragmentation factor 40 kDa subunit;DNA fragmentation factor subunit beta;DNA fragmentation factor, 40 kD, beta subunit;DNase inhibited by DNA fragmentation factor." in publications.
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What is the shipping cost for "CAD Antibody (OAPB00038)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "CAD Antibody (OAPB00038)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "CAD Antibody (OAPB00038)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "40 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "CAD Antibody (OAPB00038)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "Dffb"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "Dffb"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "Dffb"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "Dffb"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "Dffb"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "Dffb"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.