CAD Antibody (OAGA00091)

Catalog No: OAGA00091

Size:100 ul

Sample (30?g whole cell lysate)
A:293T whole cell lysate
5% SDS PAGE
OAGA00091 diluted at 1:1500

CAD antibody [C2C3], C-term detects CAD protein by Western blot analysis.
A. 30 ug C2C12 whole cell lysate/extract
5 % SDS-PAGE
CAD antibody [C2C3], C-term (OAGA00091) dilution: 1:1000

Immunohistochemical analysis of Salivary gland cancer, using CAD(OAGA00091) antibody at 1:100 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IHC-FFPE, WB
• Additional Information: Background: The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. [provided by RefSeq]
  Positive Controls: 293T, C2C12
  Cellular Localization: Cytoplasm
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1625 and 1930 of CAD (Uniprot ID#P27708)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.41 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: CAD
• Gene Full Name: CAD protein
• Alias Symbols: CDG1Z, DEE50, GATD4, EIEE50
• NCBI Gene Id: 790
• Protein Name: CAD protein
• Uniprot ID: P27708
• Protein Accession #: NP_004332.2
• Molecular Weight: 243 kDa