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Catalog No: OKCD01855 (Formerly GWB-KBBRQ2)
Size:96WELLS
Price: $675.00
SKU
OKCD01855
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Datasheets/ManualsPrintable datasheet for CACNA1D ELISA Kit (Human) (OKCD01855)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcium Channel, Voltage Dependent, L-Type, Alpha 1D Subunit (CACNa1D). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calcium Channel, Voltage Dependent, L-Type, Alpha 1D Subunit (CACNa1D). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcium Channel, Voltage Dependent, L-Type, Alpha 1D Subunit (CACNa1D), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Calcium Channel, Voltage Dependent, L-Type, Alpha 1D Subunit (CACNa1D) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calcium Channel, Voltage Dependent, L-Type, Alpha 1D Subunit (CACNa1D) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calcium Channel, Voltage Dependent, L-Type, Alpha 1D Subunit (CACNa1D) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CACNA1D Microplate96 Wells (12 x 8 Well Strips)
CACNA1D Lyophilized Standard2 x 20 ng
100X Biotinylated CACNA1D Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids
Sensitivity< 0.121 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Calcium Channel, Voltage Dependent, L-Type, Alpha 1D Subunit (CACNa1D). No significant cross-reactivity or interference between Calcium Channel, Voltage Dependent, L-Type, Alpha 1D Subunit (CACNa1D) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCACNA1D
Gene Full Namecalcium voltage-gated channel subunit alpha1 D
Alias SymbolsCACH3;CACN4;CACNL1A2;calcium channel, L type, alpha-1 polypeptide;Calcium channel, L type, alpha-1 polypeptide, isoform 2;calcium channel, neuroendocrine/brain-type, alpha 1 subunit;calcium channel, voltage-dependent, L type, alpha 1D subunit;Cav1.3;CCHL1A2;PASNA;SANDD;voltage-dependent L-type calcium channel subunit alpha-1D;voltage-gated calcium channel alpha 1 subunit;voltage-gated calcium channel alpha subunit Cav1.3;Voltage-gated calcium channel subunit alpha Cav1.3.
NCBI Gene Id776
Protein NameVoltage-dependent L-type calcium channel subunit alpha-1D
Description of TargetVoltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1D gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA).
Uniprot IDQ01668
Protein Accession #NP_000711.1
Nucleotide Accession #NM_000720.3
Protein Size (# AA)2161
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