CA9 ELISA Kit (Human) (OKCD08379)

Catalog No: OKCD08379

Size:96WELLS

CA9 ELISA Kit (Human) (OKCD08379) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carbonic Anhydrase IX (CA9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carbonic Anhydrase IX (CA9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carbonic Anhydrase IX (CA9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Carbonic Anhydrase IX (CA9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.6-1,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carbonic Anhydrase IX (CA9) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carbonic Anhydrase IX (CA9) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CA9 Microplate: 96 Wells (12 x 8 Well strips)
  • CA9 Lyophilized Standard: 2
  • 100X CA9 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity: < 2.9pg/mL

Target Info

• Gene Symbol: CA9
• Gene Full Name: carbonic anhydrase 9
• Alias Symbols: CAIX;CA-IX;carbonate dehydratase IX;carbonic anhydrase 9;carbonic anhydrase IX;carbonic dehydratase;membrane antigen MN;MN;P54/58N;pMW1;RCC-associated antigen G250;RCC-associated protein G250;renal cell carcinoma-associated antigen G250.
• NCBI Gene Id: 768
• Protein Name: Carbonic anhydrase 9
• Description of Target: Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.
• Uniprot ID: Q16790
• Protein Accession #: NP_001207.2
• Nucleotide Accession #: NM_001216.2