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Catalog No: OKCD01736 (Formerly GWB-KBBSV7)
Size:96WELLS
Price: $675.00
SKU
OKCD01736
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Datasheets/ManualsPrintable datasheet for CA6 ELISA Kit (Human) (OKCD01736)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)99-105%79-91%80-101%87-99%
EDTA Plasma (n=5)80-97%92-101%90-98%87-99%
Heparin Plasma (n=5)88-101%83-97%89-103%97-104%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carbonic Anhydrase VI (CA6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carbonic Anhydrase VI (CA6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carbonic Anhydrase VI (CA6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Carbonic Anhydrase VI (CA6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.78-50ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)98-105102
EDTA Plasma (n=5)89-9692
Heparin Plasma (n=5)98-105101
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carbonic Anhydrase VI (CA6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carbonic Anhydrase VI (CA6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CA6 Microplate96 Wells (12 x 8 Well Strips)
CA6 Lyophilized Standard2 x 20 ng
100X Biotinylated CA6 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, saliva and other biological fluids
Sensitivity< 0.131 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Carbonic Anhydrase VI (CA6). No significant cross-reactivity or interference between Carbonic Anhydrase VI (CA6) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCA6
Gene Full Namecarbonic anhydrase 6
Alias Symbolscarbonate dehydratase VI;carbonic anhydrase 6;Carbonic anhydrase VI;carbonic anhydrase VI nirs variant 2;CA-VI;GUSTIN;salivary carbonic anhydrase;secreted carbonic anhydrase.
NCBI Gene Id765
Protein NameCarbonic anhydrase 6
Description of TargetReversible hydration of carbon dioxide. Its role in saliva is unknown.
Uniprot IDP23280
Protein Accession #NP_001206.2
Nucleotide Accession #NM_001215.3
Protein Size (# AA)308
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