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Catalog No: OKCD08378
Size:96WELLS
Price: $800.00
SKU
OKCD08378
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Datasheets/ManualsPrintable datasheet for CA5B ELISA Kit (Human) (OKCD08378)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carbonic Anhydrase VB, Mitochondrial (CA5B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carbonic Anhydrase VB, Mitochondrial (CA5B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carbonic Anhydrase VB, Mitochondrial (CA5B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Carbonic Anhydrase VB, Mitochondrial (CA5B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carbonic Anhydrase VB, Mitochondrial (CA5B) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carbonic Anhydrase VB, Mitochondrial (CA5B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
CA5B Microplate96 Wells (12 x 8 Well strips)
CA5B Lyophilized Standard2
100X CA5B HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids.
Sensitivity< 0.058ng/mL
Gene SymbolCA5B
Gene Full Namecarbonic anhydrase 5B
Alias Symbolscarbonate dehydratase VB;carbonic anhydrase 5B, mitochondrial;Carbonic anhydrase VB;carbonic anhydrase VB, mitochondrial;carbonic dehydratase;CAVB;CA-VB.
NCBI Gene Id11238
Protein NameCarbonic anhydrase 5B, mitochondrial
Description of TargetReversible hydration of carbon dioxide.
Uniprot IDQ9Y2D0
Protein Accession #NP_009151.1
Nucleotide Accession #NM_007220.3
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