Catalog No: OKCD08378
Size:96WELLS
Price: $800.00
SKU
OKCD08378
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
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- $55 + $25/Kit in US
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Datasheets/Manuals | Printable datasheet for CA5B ELISA Kit (Human) (OKCD08378) |
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Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carbonic Anhydrase VB, Mitochondrial (CA5B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carbonic Anhydrase VB, Mitochondrial (CA5B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carbonic Anhydrase VB, Mitochondrial (CA5B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Carbonic Anhydrase VB, Mitochondrial (CA5B) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carbonic Anhydrase VB, Mitochondrial (CA5B) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carbonic Anhydrase VB, Mitochondrial (CA5B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Serum, plasma and other biological fluids. | ||||||||||||||||||||||
Sensitivity | < 0.058ng/mL |
Gene Symbol | CA5B |
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Gene Full Name | carbonic anhydrase 5B |
Alias Symbols | carbonate dehydratase VB;carbonic anhydrase 5B, mitochondrial;Carbonic anhydrase VB;carbonic anhydrase VB, mitochondrial;carbonic dehydratase;CAVB;CA-VB. |
NCBI Gene Id | 11238 |
Protein Name | Carbonic anhydrase 5B, mitochondrial |
Description of Target | Reversible hydration of carbon dioxide. |
Uniprot ID | Q9Y2D0 |
Protein Accession # | NP_009151.1 |
Nucleotide Accession # | NM_007220.3 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
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