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Catalog No: OKCD06808
Size:96WELLS
Price: $650.00
SKU
OKCD06808
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Datasheets/ManualsPrintable datasheet for CA2 ELISA Kit (Chicken) (OKCD06808)
Product Info
Predicted Species ReactivityChicken|Gallus gallus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carbonic Anhydrase II (CA2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carbonic Anhydrase II (CA2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carbonic Anhydrase II (CA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Carbonic Anhydrase II (CA2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range1.56-100ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carbonic Anhydrase II (CA2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carbonic Anhydrase II (CA2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
CA2 Microplate96 Wells (12 x 8 Well strips)
CA2 Lyophilized Standard2
100X CA2 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 0.71ng/mL
Gene SymbolCA2
Gene Full Namecarbonic anhydrase 2
Alias SymbolsCA-II;carbonate dehydratase II;carbonic anhydrase 2;carbonic anhydrase II.
NCBI Gene Id396257
Protein NameCarbonic anhydrase 2
Description of TargetEssential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide.
Uniprot IDP07630
Protein Accession #NP_990648.1
Nucleotide Accession #NM_205317.1
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