CA1 ELISA Kit (Bovine) (OKEH08425)

Catalog No: OKEH08425

Size:96T

Bovine CA1 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology CA1 ELISA Kit (Bovine) (OKEH08425) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CA1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CA1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CA1 captured in well.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=6.5% (n = 20)
  Mean Inter-assay CV%: <=9.0% (n = 20)
• ELISA Kit Component:
  • CA1 Microplate: 96 Wells (12 x 8 Well strips)
  • CA1 Lyophilized Standard: 2
  • 100X Biotinylated CA1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Reversible hydration of carbon dioxide.
  Subcellular Location: Cytoplasm
  Unigene: Bt.64188
  SMR: Q1LZA1
  String: 9913.ENSBTAP00000043090
  KEGG: bta:510801
  Pfam: PF00194
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.21ng/mL
• Specificity: Natural and recombinant Bovine Carbonic anhydrase 1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CA1
• Gene Full Name: carbonic anhydrase I
• Alias Symbols: CA-I;carbonate dehydratase I;carbonic anhydrase 1;Carbonic anhydrase I.
• NCBI Gene Id: 510801
• Protein Name: Q1LZA1
• Description of Target: Carbonic anhydrase 1
• Uniprot ID: https://www.uniprot.org/uniprot/Q1LZA1
• Protein Accession #: NP_001068934.1
• Nucleotide Accession #: NM_001075466.1