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Datasheets/Manuals | Printable datasheet for OAAB09214 |
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Tested Species Reactivity | Human |
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Predicted Species Reactivity | Mouse |
Product Format | Liquid. PBS with 0.09% (W/V) sodium azide |
Clonality | Polyclonal |
Isotype | Ig |
Host | Rabbit |
Application | WB |
Additional Information | Cellular Location: Nucleus. Cytoplasm. Cytoplasm, P-body. Cytoplasm, Stress granule. Endosome. Lysosome Cytoplasmic vesicle, autophagosome Secreted. Cell projection, axon. Cell projection, growth cone. Perikaryon {ECO:0000250|UniProtKB:Q6DFW0}. Note=Detected in the cytoplasm of neurons from brain tissue. Detected in the nucleus in fibroblasts. During corticogenesis, transitions from being predominantly cytoplasmic to a more even nucleocytoplasmic distribution {ECO:0000250|UniProtKB:Q6DFW0, ECO:0000269|PubMed:21944778, ECO:0000269|PubMed:21944779, ECO:0000269|PubMed:27037575} Isoform 2: Nucleus membrane; Peripheral membrane protein. Nucleus Note=Detected at the nuclear membrane of cerebellar Purkinje cells and spinal motor neurons. Also shows diffuse nuclear expression in spinal motor neurons. Tissue Location: Both isoforms are widely expressed, including kidney, lung, liver, heart, testis and several brain regions, such as cerebellum. Also expressed in the frontal cortex and in lymphoblasts (at protein level). |
Reconstitution and Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Immunogen | This C9orf72 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 396-424 amino acids from the C-terminal region of human C9orf72. |
Purification | This antibody is purified through a protein A column, followed by peptide affinity purification. |
Concentration | 0.5 mg/ml |
Intended Use | For research use only and not for use in diagnostic or therapeutic procedures. |
Application Info | WB: 1:1000 Optimal dilutions should be determined by the end user. |
Reference | Suarez-Gestal, M., et al. Arthritis Res. Ther. 12 (2), R72 (2010) : van Es, M.A., et al. Nat. Genet. 41(10):1083-1087(2009) Humphray, S.J., et al. Nature 429(6990):369-374(2004) |
Gene Symbol | C9orf72 |
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Alias Symbols | ALSFTD, DENND9, FTDALS, DENNL72, FTDALS1 |
NCBI Gene Id | 203228 |
Protein Name | Uncharacterized protein C9orf72 |
Description of Target | Component of the C9orf72-SMCR8 complex, a complex that has guanine nucleotide exchange factor (GEF) activity and regulates autophagy. In the complex, C9orf72 and SMCR8 probably constitute the catalytic subunits that promote the exchange of GDP to GTP, converting inactive GDP-bound RAB8A and RAB39B into their active GTP-bound form, thereby promoting autophagosome maturation. The C9orf72-SMCR8 complex also acts as a regulator of autophagy initiation by interacting with the ATG1/ULK1 kinase complex and modulating its protein kinase activity. Positively regulates initiation of autophagy by regulating the RAB1A-dependent trafficking of the ATG1/ULK1 kinase complex to the phagophore which leads to autophagosome formation. Acts as a regulator of mTORC1 signaling by promoting phosphorylation of mTORC1 substrates. Plays a role in endosomal trafficking. May be involved in regulating the maturation of phagosomes to lysosomes (By similarity). Regulates actin dynamics in motor neurons by inhibiting the GTP-binding activity of ARF6, leading to ARF6 inactivation. This reduces the activity of the LIMK1 and LIMK2 kinases which are responsible for phosphorylation and inactivation of cofilin, leading to cofilin activation. Positively regulates axon extension and axon growth cone size in spinal motor neurons. Plays a role within the hematopoietic system in restricting inflammation and the development of autoimmunity |
Uniprot ID | Q96LT7 |
Protein Accession # | NP_001242983.1 |
Molecular Weight | 54 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "C9orf72 Antibody - C-terminal region (OAAB09214)"?
The tested species reactivity for this item is "Human". This antibody is predicted to have homology to "Mouse".
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How long will it take to receive "C9orf72 Antibody - C-terminal region (OAAB09214)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "C9orf72 Antibody - C-terminal region (OAAB09214)" provided in?
This item is provided in "Liquid. PBS with 0.09% (W/V) sodium azide".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "C9orf72 Antibody - C-terminal region (OAAB09214)"?
This target may also be called "ALSFTD, DENND9, FTDALS, DENNL72, FTDALS1" in publications.
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What is the shipping cost for "C9orf72 Antibody - C-terminal region (OAAB09214)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "C9orf72 Antibody - C-terminal region (OAAB09214)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "C9orf72 Antibody - C-terminal region (OAAB09214)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "54 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "C9orf72 Antibody - C-terminal region (OAAB09214)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "C9ORF72"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "C9ORF72"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "C9ORF72"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "C9ORF72"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "C9ORF72"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "C9ORF72"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.