C8G Chemi-Luminescent ELISA Kit (Human) (OKCD03351)

Catalog No: OKCD03351

Size:96WELLS

Human C8G ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	92-99%	78-90%	93-101%	79-101%
  EDTA Plasma (n=5)	82-101%	90-99%	91-105%	83-96%
  Heparin Plasma (n=5)	98-105%	92-99%	97-105%	80-101%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Complement Component 8g (C8g). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Complement Component 8g (C8g). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Complement Component 8g (C8g) level in the sample or standard.
• ELISA Kit Range: 6.86-5,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	96-104	101
  EDTA Plasma (n=5)	85-101	96
  Heparin Plasma (n=5)	83-103	93

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Component 8g (C8g) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Component 8g (C8g) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • C8G Microplate: 96 Wells (12 x 8 Well strips)
  • C8G Lyophilized Standard: 2
  • 100X C8G Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.078 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: C8G
• Gene Full Name: complement C8 gamma chain
• Alias Symbols: C8C;complement component 8, gamma polypeptide;complement component C8 gamma chain.
• NCBI Gene Id: 733
• Protein Name: Complement component C8 gamma chain
• Description of Target: C8 is a constituent of the membrane attack complex. C8 binds to the C5B-7 complex, forming the C5B-8 complex. C5-B8 binds C9 and acts as a catalyst in the polymerization of C9. The gamma subunit seems to be able to bind retinol.
• Uniprot ID: P07360
• Protein Accession #: NP_000597
• Nucleotide Accession #: NM_000606