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Datasheets/Manuals | Printable datasheet for C5 Antibody (OASA01017) |
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Predicted Species Reactivity | Human |
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Product Format | Liquid. Borate buffered saline |
Clonality | Monoclonal |
Clone | 046-10.2.2 (10-04) |
Isotype | IgG1 |
Host | Mouse |
Application | Enzyme-linked immunosorbent assay|Functional assay|Western blot |
:: | Preparation: Purified IgG prepared by affinity chromatography on Protein A Preservative Stabilisers: 0.1% - Sodium Azide (NaN3) |
:: | Approx Protein Conc: IgG concentration 1.0mg/ml Buffer Solutions: Borate buffered saline |
Reconstitution and Storage | -20°C |
Immunogen | Purified human C5. |
Purification | Purified IgG prepared by affinity chromatography on Protein A from ascites |
Predicted Homology Based on Immunogen Sequence | Human |
Concentration | 1.08 mg/ml |
Specificity | OASA01017 recognises secreted complement component 5 (C5), a 190kDa serine protease present in blood serum. It is composed of an 115kDa disulphide linked alpha-chain and a 75 kDa beta-chain. In addition to its role in complement, C5 is thought to be associated with inflammation, stimulating tumour necrosis factor alpha, interferon gamma mediated suppression of IL10 and IL12 production. C5 is activated via either the classical or the alternative complement pathway, by proteolytic cleavage of C5a from the N-terminal of the alpha-chain, by C5 convertase. C5a is an anaphylotoxin which mediates local inflammation. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. It also stimulates chemotaxis of neutrophils, eosinophils, basophils and monocytes towards the site of inflammation. The remaining fragment, C5b, initiates the assembly of the membrane attack complex. In haemolysis inhibition studies it was found that OASA01017 will block C5 haemolytic activity. Removal of sodium azide is recommended prior to use in functional assays. Aviva recommend the use of OASA11305 for this purpose. |
Reference | 1. Karp, C. et al. (2000) Identification of complement factor 5 as a susceptibility locus for experimental allergic asthma. Nature Immun. 1: 221-226. 2. Peng, T. et al. (2005) Role of C5 in the development of airway inflammation, airway hyperresponsiveness, and ongoing airway response. J. Clin. Invest. 115: 1590-1600. |
Gene Symbol | CPAMD4, FLJ17816, FLJ17822, MGC142298, C5 |
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Alias Symbols | C5D, C5a, C5b, ECLZB, CPAMD4 |
NCBI Gene Id | 727 |
Protein Name | Complement C5 |
Description of Target | MOUSE ANTI HUMAN C5 |
Uniprot ID | P01031 |
Protein Accession # | NP_001726.2 |
Protein Size (# AA) | 1676 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "C5 Antibody (OASA01017)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "C5 Antibody (OASA01017)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "C5 Antibody (OASA01017)" provided in?
This item is provided in "Liquid. Borate buffered saline".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "C5 Antibody (OASA01017)"?
This target may also be called "C5D, C5a, C5b, ECLZB, CPAMD4" in publications.
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What is the shipping cost for "C5 Antibody (OASA01017)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "C5 Antibody (OASA01017)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "C5 Antibody (OASA01017)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "C5 Antibody (OASA01017)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "CPAMD4"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "CPAMD4"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "CPAMD4"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "CPAMD4"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "CPAMD4"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "CPAMD4"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.