C4BPA ELISA Kit (Human) : 96 Wells (OKEH00553)

Catalog No: OKEH00553

Size:96T

Human C4BPA ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology C4BPA ELISA Kit (Human) (OKEH00553) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human C4BPA has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for Human C4BPA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human C4BPA captured in well.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 103%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 7.3% (n = 20)
  Mean Inter-assay CV%: < 10.4% (n = 20)
• ELISA Kit Component:
  • C4BPA Microplate: 96 Wells (12 x 8 Well strips)
  • C4BPA Lyophilized Standard: 2
  • 100X Biotinylated C4BPA Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.56 ng/mL
• Specificity: Natural and recombinant Human C4b-binding protein alpha chain
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: C4BPA
• Gene Full Name: complement component 4 binding protein alpha
• Alias Symbols: C4b-binding protein alpha chain;C4BP;proline-rich protein;PRP.
• NCBI Gene Id: 722
• Protein Name: P04003
• Description of Target: C4b-binding protein alpha chain
• Uniprot ID: https://www.uniprot.org/uniprot/P04003
• Protein Accession #: NP_000706.1
• Nucleotide Accession #: NM_000715.3
• Protein Size (# AA): 597
• Molecular Weight: 67 kDa