C4b ELISA Kit (Mouse) (OKCD01577)

Catalog No: OKCD01577 (Formerly GWB-KBBFX2)

Size:96WELLS

Mouse C4b ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	78-89%	92-99%	97-105%	97-105%
  EDTA Plasma (n=5)	81-105%	96-104%	81-103%	84-99%
  Heparin Plasma (n=5)	94-103%	82-93%	83-92%	89-102%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement C4-B (C4B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement C4-B (C4B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement C4-B (C4B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Complement C4-B (C4B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	85-104	96
  EDTA Plasma (n=5)	95-102	98
  Heparin Plasma (n=5)	99-105	102

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement C4-B (C4B) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement C4-B (C4B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-C4b Microplate: 96 Wells (12 x 8 Well Strips)
  • C4b Lyophilized Standard: 2 x 1,000 ng
  • 100X Biotinylated C4b Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: < 6.7 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Complement Component 4b (C4b). No significant cross-reactivity or interference between Complement Component 4b (C4b) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: C4b
• Gene Full Name: complement component 4B (Chido blood group)
• Alias Symbols: C4;complement C4-B;complement component 4 (within H-2S);complement component 4B (Childo blood group);Ss.
• NCBI Gene Id: 12268
• Protein Name: Complement C4-B
• Description of Target: Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. Catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens (By similarity).
• Uniprot ID: P01029
• Protein Accession #: NP_033910.2
• Nucleotide Accession #: NM_009780.2
• Protein Size (# AA): 1738