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Datasheets/Manuals | Printable datasheet for anti-C4B (OALA02951) antibody |
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Tested Species Reactivity | Human |
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Predicted Species Reactivity | Human |
Product Format | Liquid. PBS with 0.02% sodium azide. |
Clonality | Polyclonal |
Isotype | IgY |
Host | Chicken |
Conjugation | Unconjugated |
Application | ELISA, FC, IHC, IHC-P, WB |
Reconstitution and Storage | Store at 4C for short term applications. For long term storage, aliquot and store at -20C. |
Immunogen | Purified human complement C4b |
Purification | Purified |
Concentration | 1 mg/ml |
Specificity | Anti-Human Complement Component C4b polyclonal antibody reacts with human C4b. C4 is a 210 KDa trimeric glycoprotein containing three polypeptide chains alpha, beta, and gamma with respective molecular weights of 93KDa, 75KDa and 33 KDa. C4 breaks down in. |
Intended Use | For research use only. |
Application Info | ELISA, Flo (0.1 ug/10E6 cells), IHC, IHC-P (20 ug/ml), WB |
Application Data | Immunohistochemistry: OALA02951 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for OALA02951 was determined to be 20 ug/ml. |
Storage Buffer | PBS, 0.02% Sodium Azide |
Gene Symbol | C4B |
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Gene Full Name | Complement C4-B |
Alias Symbols | C4B, C4B12, C4BD, C4B1, C4B3, Complement C4B, CH, Chido form of C4, CPAMD3, Complement C4-B, CO4, Basic complement C4, C4B2, C4B5, C4F, Complement C4B1a, Complement component 4B |
NCBI Gene Id | 721 |
Protein Name | Complement C4-B |
Description of Target | Non-enzymatic component of the C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. |
Uniprot ID | P0C0L5 |
Protein Accession # | NP_001002029.3; NP_001229752.1 |
Nucleotide Accession # | NM_001002029.3; NM_001242823.2 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "C4B Antibody (OALA02951)"?
The tested species reactivity for this item is "Human". This antibody is predicted to have homology to "Human".
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How long will it take to receive "C4B Antibody (OALA02951)"?
This item is available "Domestic: within 1 week delivery | International: 1 week".
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What buffer format is "C4B Antibody (OALA02951)" provided in?
This item is provided in "Liquid. PBS with 0.02% sodium azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "C4B Antibody (OALA02951)"?
This target may also be called "C4B, C4B12, C4BD, C4B1, C4B3, Complement C4B, CH, Chido form of C4, CPAMD3, Complement C4-B, CO4, Basic complement C4, C4B2, C4B5, C4F, Complement C4B1a, Complement component 4B" in publications.
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What is the shipping cost for "C4B Antibody (OALA02951)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "C4B Antibody (OALA02951)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "C4B Antibody (OALA02951)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "C4B Antibody (OALA02951)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "C4B"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "C4B"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "C4B"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "C4B"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "C4B"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "C4B"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.