C4A ELISA Kit (Rat) : 96 Wells (OKEH00414)

Catalog No: OKEH00414

Size:96T

Rat C4A ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology C4A ELISA Kit (Rat) (OKEH00414) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Rat C4A has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for Rat C4A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Rat C4A captured in well.
• ELISA Kit Range: 0.156-10ug/ml
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 93%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 7.2% (n = 20)
  Mean Inter-assay CV%: < 9.6% (n = 20)
• ELISA Kit Component:
  • Anti-C4A Microplate: 96 Wells (12 x 8 Well strips)
  • C4A Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated C4A: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.082ug/mL
• Specificity: Natural and recombinant Rat Complement C4
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: C4a
• Gene Full Name: complement C4A
• Alias Symbols: C4;C4-1;C4b;complement C4;complement C4-like;complement component 4 (within H-2S);complement component 4, gene 1;complement component 4A (Rodgers blood group);complement component 4B (Chido blood group);complement component 4B (Childo blood group).
• NCBI Gene Id: 24233
• Protein Name: P08649
• Description of Target: Complement C4
• Uniprot ID: https://www.uniprot.org/uniprot/P08649
• Protein Accession #: NP_113692.2
• Nucleotide Accession #: NM_031504.3
• Protein Size (# AA): 1737
• Molecular Weight: 192 kDa