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- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
| Datasheets/Manuals | Printable datasheet for C4A Antibody (OASA01014) |
|---|
| Predicted Species Reactivity | Human |
|---|---|
| Product Format | Liquid. Borate buffered saline |
| Clonality | Monoclonal |
| Clone | 10-11 |
| Isotype | IgG1 |
| Host | Mouse |
| Application | Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Frozen|Immunohistochemistry-Paraffin|Western blot |
| Reconstitution and Storage | -20°C |
| Immunogen | Native, from human plasma |
| Purification | Purified IgG prepared by Fast protein liquid chromatography (FPLC) from ascites |
| Concentration | 1 mg/ml |
| Specificity | Mouse anti Human C4d antibody, clone 10-11 recognizes the secreted protein complement component 4d (C4d). |
| Application Info | IHC-F: 1/100 - 1/750 ELISA: 1/5000 - 1/20000 IF: 1/250 - 1/600 IHC-P: It has been reported that this antibody works very well on acetone-fixed, frozen renal biopsies. Strong staining is observed in the glomeruli and in some cases the peritubular capillaries. Clone 10-11 has given variable results on formalin-fixed paraffin-embedded sections. It has been observed that pre-treatment with 88% formic acid for 20 mins at room temperature is beneficial. |
| Reference | 1. Mauiyyedi, S. et al. (2002) Acute humoral rejection in kidney transplantation: II. Morphology, immunopathology, and pathologic classification. J Am Soc Nephrol. 13: 779-787. 2. Collins, A.B. et al. (1999) Complement activation in acute humoral renal allograft rejection: diagnostic |
| Gene Symbol | C4A |
|---|---|
| Alias Symbols | C4, RG, C4S, CO4, C4A2, C4A3, C4A4, C4A6, C4AD, CPAMD2 |
| NCBI Gene Id | 720 |
| Protein Name | Complement C4-A |
| Description of Target | Mouse anti Human C4d antibody, clone 10-11 is used to detect the biomarker C4d which has been described as a “footprint” of antibody mediated tissue rejection. The internal thioester of C4b becomes exposed during cleavage to C4d and forms a covalent bond with the cell surface. The longer half-life of covalently bound C4d makes it a footprint of complement activation long after weakly bound antibodies have been cleared by the blood stream |
| Uniprot ID | P0C0L4 |
| Protein Accession # | NP_009224.2 |
| Protein Size (# AA) | 1744 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "C4A Antibody (OASA01014)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "C4A Antibody (OASA01014)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "C4A Antibody (OASA01014)" provided in?
This item is provided in "Liquid. Borate buffered saline".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "C4A Antibody (OASA01014)"?
This target may also be called "C4, RG, C4S, CO4, C4A2, C4A3, C4A4, C4A6, C4AD, CPAMD2" in publications.
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What is the shipping cost for "C4A Antibody (OASA01014)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "C4A Antibody (OASA01014)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "C4A Antibody (OASA01014)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "C4A Antibody (OASA01014)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "C4A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "C4A"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "C4A"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "C4A"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "C4A"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "C4A"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
